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首页> 外文期刊>International Current Pharmaceutical Journal >Screening, isolation and purification of antibacterial agents from marine actinomycetes
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Screening, isolation and purification of antibacterial agents from marine actinomycetes

机译：从海洋放线菌中筛选，分离和纯化抗菌剂

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As marine environmental conditions are extremely different from terrestrial ones, it is surmised that marine actinomycetes might produce novel bioactive compounds. Hence marine sediments, collected from the coastal areas of Gokharna and Muradeshwara of Karnataka state, were screened Seventeen isolates were obtained on starch-casein agar media by soil dilution technique. However, only six isolates namely ACT-A2, ACT-A3, ACT-A4, ACT-A5, ACT-A7 and ACT-A15 showed significant antibacterial activity against both gram-positive and gram-negative bacteria. Morphological, cultural and biochemical characterization indicated that the isolates belong to Streptomyces genus of Actinomycetes. Further studies were carried out with the most active ACT-A2. Optimization of media, temperature and pH by shake flask fermentation indicated starch-casein, 28°C and 7 to be suitable for ACT-A2. The production of antibiotics began after 24 h, reached maximum at 72 h and maintained at the same level up to 120 h. Ethyl acetate was used to extract antibacterial compounds from the culture filtrate. TLC was done on silica gel using ethyl acetate: methanol (6:4) and direct bioautography has shown the presence of two active substance, one with Rf 0.8 has more activity than the other with Rf 0.4. Further purification is done by column chromatography using a mixture of dicholoromethane and ethyl acetate. The findings from this investigation reveal that the strain ACT-A2 in order exhibited superior antimicrobial activities to other sediment isolates of actinomycetes.DOI:?http://dx.doi.org/10.3329/icpj.v1i12.12448 International Current Pharmaceutical Journal 2012, 1(12): 394-402
机译：由于海洋环境条件与陆地环境极为不同，因此推测海洋放线菌可能会产生新的生物活性化合物。因此，从土壤中提取了从卡纳塔克邦Gokharna和Muradeshwara沿海地区收集的海洋沉积物，在淀粉-酪蛋白琼脂培养基上获得了17种分离物。但是，只有六个分离株，即ACT-A2，ACT-A3，ACT-A4，ACT-A5，ACT-A7和ACT-A15对革兰氏阳性和革兰氏阴性细菌均表现出显着的抗菌活性。形态，文化和生化特征表明，分离物属于放线菌的链霉菌属。用最活跃的ACT-A2进行了进一步的研究。通过摇瓶发酵的培养基，温度和pH的优化表明淀粉-酪蛋白，28℃和7适合于ACT-A2。抗生素的生产在24小时后开始，在72小时达到最高，并在120小时内保持相同水平。乙酸乙酯用于从培养滤液中提取抗菌化合物。 TLC在硅胶上使用乙酸乙酯：甲醇（6：4）进行，直接生物自显影显示了两种活性物质的存在，一种具有Rf 0.8的活性高于另一种具有Rf 0.4的活性。使用二氯甲烷和乙酸乙酯的混合物，通过柱色谱法进一步纯化。这项调查的结果表明，菌株ACT-A2表现出比放线菌的其他沉积物分离物优越的抗菌活性.DOI：？http：//dx.doi.org/10.3329/icpj.v1i12.12448 International Current Pharmaceutical Journal 2012 ，1（12）：394-402

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