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首页> 外文期刊>International Journal of Agricultural and Biosystems Engineering >Antimicrobial Activities for Green Synthesis of Silver Nanoparticles Using Stevia rebaudiana and Pluchea dioscoridis Leaves
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Antimicrobial Activities for Green Synthesis of Silver Nanoparticles Using Stevia rebaudiana and Pluchea dioscoridis Leaves

机译：甜叶菊和Pluchea dioscoridis叶子对绿色合成银纳米粒子的抗菌活性

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Stevia rebaudiana aqueous extract (St) and Pluchea dioscoridis aqueous extract (Pd) with different volumes (0.2 and 0.4 ml) and (1.0 and 2.0 ml) were used to synthesize silver nanoparticles (AgNPs) under dark and light, respectively. The color of the solutions was yellowish to reddish brown. The surface plasmon resonance (SPR) band of AgNPs synthesized by St (0.2 ml) appeared between 422-565 nm and 437-573 nm in dark and light conditions, respectively. While, AgNPs formed by St (0.4 ml) gave SPR band between 439-482 nm in darkness and 442-532 nm in lightness. Also, the maximum peak of AgNPs synthesized by Pd (1 ml) was around 445.5 nm in dark and 450.5 nm in light. Furthermore, a broad peak was observed in the result of utilizing Pd (2 ml) which had a maximum peak at 452 and 450.5 in darkness and lightness, respectively. The broad peak illustrates that the AgNPs are polydispersed which agrees with transmission electron microscope (TEM) analysis. All microbial strains (Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Candida utilis) were inhibited with AgNPs formed using St in both concentrations and in both light and dark. On the other hand, AgNPs synthesized by Pd in both concentrations and in both light and dark were had a high inhibition effect in B. subtilis and E. coli.
机译：用不同体积（0.2和0.4 ml）和（1.0和2.0 ml）的甜叶菊甜叶菊水提取物（St）和百日草Pluchea dioscoridis水提取物（Pd）分别在暗和亮条件下合成银纳米颗粒（AgNPs）。溶液的颜色为淡黄色至红棕色。由St（0.2 ml）合成的AgNPs的表面等离振子共振（SPR）带分别出现在黑暗和明亮条件下的422-565 nm和437-573 nm之间。同时，由St（0.4 ml）形成的AgNPs在黑暗中的SPR带在439-482 nm之间，在明亮度在442-532 nm之间。同样，由钯（1 ml）合成的AgNPs的最大峰在黑暗中约为445.5 nm，在光中约为450.5 nm。此外，在使用Pd（2ml）的结果中观察到一个宽峰，该Pd在黑暗和明暗中分别在452和450.5处具有最大峰。宽峰表明AgNPs是多分散的，与透射电子显微镜（TEM）分析一致。所有微生物菌株（枯草芽孢杆菌，金黄色葡萄球菌，大肠杆菌和假丝酵母）均被浓度由St形成的AgNPs抑制，在浓度和明暗情况下均如此。另一方面，由Pd合成的AgNPs在浓度和浓度下均在枯草芽孢杆菌和大肠杆菌中具有很高的抑制作用。

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