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首页> 外文期刊>International Journal of Biomedical Science >Simultaneous Determination of Miconazole Nitrate and Metronidazole in Different Pharmaceutical Dosage Forms by Gas Chromatography and Flame Ionization Detector (GC-FID)
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Simultaneous Determination of Miconazole Nitrate and Metronidazole in Different Pharmaceutical Dosage Forms by Gas Chromatography and Flame Ionization Detector (GC-FID)

机译：气相色谱和火焰电离检测器（GC-FID）同时测定不同药物剂型中的硝酸咪康唑和甲硝唑

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摘要

A simple, rapid and precise gas chromatographic method has been developed for the simultaneous determination of miconazole nitrate (MIZ) and metronidazole (MNZ) in tablets and ovules, using a capillary column AE.SE-54 (15 m × 0.53 mm, i.d.) and nitrogen as a carrier gas at a flow rate of 9 mL min-1. The oven temperature was programmed at 140°C for 3 min, with a rise of 40°C min-1 up to 180°C (held for 2 min) and then increased to a final temperature of 250°C. The injector and detector port temperatures were maintained at 260°C. Detection was carried out using flame ionization detector. Results of assay and recovery studies were statistically evaluated for its accuracy and precision. The retention times were about 3.50 and 12.90min for MNZ and MIZ, respectively. Linearity ranges were 50.0-6030.0 and 62.5-2000.0 μg mL-1 for MNZ and MIZ, with limit of detection values of 2.5 and 3.1 μg mL-1, respectively. Correlation coefficients (R2) of the regression equations were greater than 0.999 in all cases. No interference from any components of pharmaceutical dosage forms or degradation products was observed. According to the validation results, the proposed method was found to be specific, accurate, precise and could be applied to the simultaneous quantitative analysis of MIZ and MNZ in tablets and ovules.
机译：开发了一种简单，快速，精确的气相色谱方法，使用毛细管柱AE.SE-54（15 m×0.53 mm，内径）同时测定片剂和胚珠中的硝酸咪康唑（MIZ）和甲硝唑（MNZ）氮气作为载气，流速为9 mL min-1。将烤箱温度设定为140°C 3分钟，然后将40°C min-1升高到180°C（保持2分钟），然后升高到250°C的最终温度。进样器和检测器端口的温度保持在260°C。使用火焰离子化检测器进行检测。对测定和回收率研究的结果进行统计学评估，以评估其准确性和准确性。对于MNZ和MIZ，保留时间分别约为3.50和12.90min。 MNZ和MIZ的线性范围分别为50.0-6030.0和62.5-2000.0μgmL-1，检测极限分别为2.5和3.1μgmL-1。在所有情况下，回归方程的相关系数（R2）均大于0.999。没有观察到药物剂型或降解产物的任何成分的干扰。根据验证结果，所提出的方法具有特异性，准确性，精确性，可用于片剂和胚珠中MIZ和MNZ的同时定量分析。

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