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首页> 外文期刊>International Journal of Clinical and Experimental Pathology >Anticancer effect of salidroside on colon cancer through inhibiting JAK2/STAT3 signaling pathway
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Anticancer effect of salidroside on colon cancer through inhibiting JAK2/STAT3 signaling pathway

机译：红景天苷通过抑制JAK2 / STAT3信号通路对结肠癌的抗癌作用

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Salidroside is considered to have anti-tumor properties. We investigate its effects on colon carcinoma SW1116 cells. Cell viability was assessed by CCK-8. Propidium iodide (PI) staining was used to determine the cell cycle by flow cytometry. The migration and invasion were detected by Transwell. Western blot was used to detect the expression of STAT3 signal related proteins. As the result, high concentrations of salidroside (10, 20. 50 μg/ml) significantly inhibited proliferation of SW1116 cells in a parallelly, cell cycle arrest was increased at the G0/G1 phase after salidroside treatment. Furthermore, salidroside inhibited migration and invasion of SW1116 cells. Salidroside treatment decreased proteins expression of phosphorylation levels in JAK2/STAT3 signaling, while MMP-2 and MMP-9 proteins levels were decreased and protein expression of VEGF and VEGFR-2 were down-regulated. In Conclusion, salidroside inhibited proliferation, decreased the migration and invasion of SW1116 cells in JAK2/STAT3-dependent pathway, the specific mechanisms need further study.
机译：红景天苷被认为具有抗肿瘤特性。我们调查其对结肠癌SW1116细胞的影响。通过CCK-8评估细胞活力。碘化丙啶（PI）染色用于通过流式细胞仪确定细胞周期。 Transwell检测到了迁移和入侵。 Western印迹用于检测STAT3信号相关蛋白的表达。结果，高浓度的红景天苷（10、20。50μg/ ml）平行地显着抑制SW1116细胞的增殖，红景天苷处理后G0 / G1期细胞周期停滞增加。此外，红景天苷抑制SW1116细胞的迁移和侵袭。红景天苷处理降低了JAK2 / STAT3信号转导中磷酸化水平的蛋白质表达，而MMP-2和MMP-9蛋白质水平降低，而VEGF和VEGFR-2的蛋白质表达下调。结论红景天苷可抑制JAK2 / STAT3通路中SW1116细胞的增殖，减少其迁移和侵袭，其具体机制有待进一步研究。

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