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首页> 外文期刊>International Journal of Clinical and Experimental Pathology >Expression of MMP-9/TIMP-2 in nasal polyps and its functional implications
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Expression of MMP-9/TIMP-2 in nasal polyps and its functional implications

机译：MMP-9 / TIMP-2在鼻息肉中的表达及其功能意义

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Nasal polyps (NP) involve tissue repair and structural remodel, both of which require the extracellular matrix. Matrix metalloproteinase (MMP) and tissue inhibitor of metalloproteinase (TIMP) are known regulators for tissue reconstruction. This study therefore aimed to analyze the expressional profile of MMP-9 and TIMP-2 in NP patients, with further investigation of their roles in pathogenesis. A total of 60 NP tissue samples (including 15 type I, 21 type II and 24 type III) were collected from surgeries in our hospital, in addition to 6 normal ethmoid sinus mucosa samples. The mRNA and protein expression levels of MMP-9/TIMP-2 were quantified by real-time PCR and Western blotting, respectively. Serum levels were also checked by enzyme-linked immunosorbent assay (ELISA). Both mRNA and protein levels of MMP-9 in NP tissues or serum were significantly elevated compared to those in control ones (P<0.05) while the TIMP-2 expression was suppressed (P<0.05). In patients with more advanced stage, MMP-9 expression was further elevated, with lowered TIMP-2 levels (P<0.05 in both cases). Pathogenesis and progression of NP is closely related with elevated MMP-9 and suppressed TIMP-2 expression, suggesting the role of those factors as indexes for evaluating NP stage. Our results also provide evidences for further studies of pathogenesis and drug targets of NP.
机译：鼻息肉（NP）涉及组织修复和结构重塑，这两者都需要细胞外基质。基质金属蛋白酶（MMP）和金属蛋白酶组织抑制剂（TIMP）是用于组织重建的已知调节剂。因此，本研究旨在分析NP患者中MMP-9和TIMP-2的表达情况，并进一步研究其在发病机制中的作用。除了6个正常筛窦窦黏膜样本外，我院共收集了60个NP组织样本（包括15个I型，21个II型和24个III型）。 MMP-9 / TIMP-2的mRNA和蛋白表达水平分别通过实时PCR和Western blotting进行定量。还通过酶联免疫吸附测定（ELISA）检查血清水平。与对照组相比，NP组织或血清中MMP-9的mRNA和蛋白水平均显着升高（P <0.05），而TIMP-2表达受到抑制（P <0.05）。在晚期患者中，MMP-9表达进一步升高，TIMP-2水平降低（两种情况均P <0.05）。 NP的发病机制和进展与MMP-9升高和TIMP-2表达抑制密切相关，提示这些因素作为评估NP阶段指标的作用。我们的结果也为进一步研究NP的发病机制和药物靶标提供了证据。

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