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首页> 外文期刊>International Journal of Electrochemical Science >Electrochemical DNA Biosensor Based on Graphene Oxide- Chitosan Hybrid Nanocomposites for Detection of Escherichia Coli O157:H7
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Electrochemical DNA Biosensor Based on Graphene Oxide- Chitosan Hybrid Nanocomposites for Detection of Escherichia Coli O157:H7

机译：基于氧化石墨烯-壳聚糖杂化纳米复合材料的电化学DNA生物传感器检测大肠杆菌O157：H7

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摘要

In this article, we present a simple and sensitive electrochemical DNA biosensor based on graphene oxide (GO)/chitosan (CS) hybrid nanocomposites modified glassy carbon electrode (GCE) for detection of Escherichia coli O157:H7 (E.coli O157:H7). The morphology and composition of GO and hybrid nanocomposites were characterized by transmission electron microscope (TEM), X-ray powder diffraction (XRD), field emission scanning electron microscopy (FESEM), and Fourier transform infrared spectrum (FTIR). Cyclic voltammetry investigations indicated that the GO/CS/GCE showed excellent electron transfer ability and good linear relation. Under the optimal hybridization conditions, electrochemical impedance spectroscopy (EIS) responses of ssDNA/GO/CS/GCE biosensor were in linear with the target DNA in the concentration range from 1×10-14 to 1×10-8 M with the detection limit as 3.584×10-15 M (3σ). Moreover, differential pulse voltammetry (DPV) studies revealed good specificity and excellent ability of ssDNA/GO/CS/GCE biosensor to distinguish complementary, 1- base mismatched DNA, 2-base mismatched DNA and multi-base mismatched DNA. The developed strategy in this research revealed that the GO/CS modified electrode possess excellent performance for detecting of Escherichia coli O157:H7 DNA.
机译：在本文中，我们提出了一种基于氧化石墨烯（GO）/壳聚糖（CS）杂化纳米复合修饰玻碳电极（GCE）的简单而灵敏的电化学DNA生物传感器，用于检测大肠杆菌O157：H7（E.coli O157：H7） 。通过透射电子显微镜（TEM），X射线粉末衍射（XRD），场发射扫描电子显微镜（FESEM）和傅里叶变换红外光谱（FTIR）对GO和杂化纳米复合材料的形貌和组成进行了表征。循环伏安法研究表明，GO / CS / GCE具有出色的电子传递能力和良好的线性关系。在最佳杂交条件下，ssDNA / GO / CS / GCE生物传感器的电化学阻抗谱（EIS）响应与目标DNA呈线性关系，浓度范围为1×10-14至1×10-8 M，且具有检测限为3.584×10-15 M（3σ）。此外，差分脉冲伏安法（DPV）研究显示ssDNA / GO / CS / GCE生物传感器具有良好的特异性和出色的能力，可以区分互补的1-碱基错配DNA，2-碱基错配DNA和多碱基错配DNA。这项研究中开发的策略表明，GO / CS修饰电极在检测大肠杆菌O157：H7 DNA方面具有出色的性能。

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