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首页> 外文期刊>International Journal of Electrochemical Science >Electrochemical and Spectroscopic Studies on the Interaction Modes of Calf Thymus DNA with Antibacterial Schiff Bases obtained from Substituted Salicylaldehydes and Sulfamethizole
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Electrochemical and Spectroscopic Studies on the Interaction Modes of Calf Thymus DNA with Antibacterial Schiff Bases obtained from Substituted Salicylaldehydes and Sulfamethizole

机译：小牛胸腺DNA与取代水杨醛和磺胺甲二唑的抗菌席夫碱相互作用模式的电化学和光谱研究

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In this study, two Schiff bases have been prepared from the condensation of sulfamethizole (SMTZ)with 3-methoxy and 5-nitro derivatives of salicylaldehyde. These compounds have been characterizedby elemental analysis, FT-IR, UV-Vis, 1H-NMR, melting point and X-ray measurements. The in vitroanti-bacterial properties of these Schiff bases against various microorganisms (E. coli ATCC 25922, S.aureus ATCC 25923 and trimethoprim sulfamethoxazole resistant clinical isolate E. coli (SXT-R E.coli)) have been also investigated. The Schiff base with -NO2 (Schiff base B, MIC: 0.5 μg mL?1)shows stronger antibacterial activity than SMTZ, other reactive compounds and the Schiff base with -OCH3 (Schiff base A) against S. aureus ATCC 25923. The interaction of the Schiff bases with calfthymus DNA (CT-DNA) in the physiological pH (7.4) was studied by electrochemical andspectroscopic methods. The electrochemical and spectroscopic data revealed that Schiff bases bind toCT-DNA in 1:1 stoichiometry. The binding affinity followed the order Schiff base A > Schiff base B.It has been found that the binding affinity orders determined from different methods are in goodagreement with each other. The obtained results indicate that Schiff base A binds to CT-DNA bymeans of electrostatic forces; however, Schiff base B could interact with CT-DNA molecule byintercalative mode.
机译：在这项研究中，由磺胺甲二唑（SMTZ）与水杨醛的3-甲氧基和5-硝基衍生物缩合制备了两个席夫碱。这些化合物已通过元素分析，FT-IR，UV-Vis，1H-NMR，熔点和X射线测量进行了表征。还研究了这些席夫碱对各种微生物的体外抗菌特性（大肠杆菌ATCC 25922，金黄色葡萄球菌ATCC 25923和耐甲氧苄啶磺胺甲基异恶唑的临床分离大肠杆菌（SXT-R大肠杆菌））。具有-NO2的席夫碱（席夫碱B，MIC：0.5μgmL？1）显示出比SMTZ，其他反应性化合物和具有-OCH3的席夫碱（席夫碱A）对金黄色葡萄球菌ATCC 25923更强的抗菌活性。相互作用用电化学和光谱法研究了在pH为7.4的小牛磺酸（CT-DNA）中希夫碱的含量。电化学和光谱数据表明，席夫碱以1：1的化学计量比与CT-DNA结合。结合亲和力遵循希夫碱A>希夫碱B的顺序。已经发现，由不同方法确定的结合亲和力顺序彼此一致。结果表明，席夫碱A通过静电力与CT-DNA结合。但是，席夫碱B可以通过插入模式与CT-DNA分子相互作用。

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