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首页> 外文期刊>International Journal of Electrochemical Science >Novel approach to fabrication of DNA Biosensor Based on a Carboxylated Graphene Oxide Decorated with Fe3O4 NPs for the Detection of Typhoidal Salmonella
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Novel approach to fabrication of DNA Biosensor Based on a Carboxylated Graphene Oxide Decorated with Fe3O4 NPs for the Detection of Typhoidal Salmonella

机译：Fe 3 O 4 NPs修饰的羧化氧化石墨烯制备DNA生物传感器的新方法用于检测伤寒沙门氏菌

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Diseases caused by typhoidal salmonella have led to a wide range of panic. It is thereby criticallyurgent to develop high-efficiency, ultrasensitive techniques for typhoidal salmonella detection.Carboxylated graphene oxide (CGO), features as one of the most crucial substrates to immobilizeprobe DNA for the fabrication of DNA biosensor, has been studied extensively so far. However, thereagents involved in the protocols proposed previously due to their strong alkalinity and extremelyhigh reducing properties would impose significant impact to their performance and application.Herein, we develop a novel strategy for effective carboxylation graphene oxide with sodium citrate andchloroacetic acid. X-ray photoelectron spectroscopy (XPS) results demonstrate the density of carboxylis improved more than twice, in consistent with the results of nuclear magnetic resonance (NMR).Furthermore, decorated with cost-effective Fe3O4 NPs, the conductivity of CGO could be enhancedremarkably, furthermore, far more 5’-end amino labeled probe DNA would be stably grafted. Theperformance of stepwise modification of the bioelectrodes is carried out by cyclic voltammetry (CV)and electrochemical impedance spectroscopes (EIS). Differential pulse voltammetry (DPV) studiesexhibit a linear response ranging from 10-17 to 10-9 M for target DNA analysis with the detection limitup to 3.16?10-18 M, using methylene blue (MB) as a redox indicator under optimal conditions. Inaddition, the as-prepared biosensor provides predominant capability for discriminating the similartarget DNA sequences. Eventually, the proposed biosensor is surveyed by real samples and it showsoutstanding performance. Consequently, the developed ssDNA/Fe3O4 NPs/CGO/GCE can be apromising candidate for real patient samples analysis.
机译：伤寒沙门氏菌引起的疾病引起了广泛的恐慌。因此，迫切需要开发一种高效，超灵敏的技术来检测伤寒沙门氏菌。迄今为止，广泛研究了羧化氧化石墨烯（CGO），它是固定探针DNA的最重要底物之一，用于制备DNA生物传感器。然而，由于其强碱性和极高的还原性能，先前提出的方案中涉及的试剂对其性能和应用将产生重大影响。在此，我们开发了一种新的策略以柠檬酸钠和氯乙酸有效地羧化氧化石墨烯。 X射线光电子能谱（XPS）结果表明，羧基的密度提高了两倍以上，与核磁共振（NMR）结果一致。此外，用具有成本效益的Fe3O4 NPs装饰，CGO的电导率可以显着提高，而且，将稳定地移植更多的5'端氨基标记的探针DNA。通过循环伏安法（CV）和电化学阻抗谱仪（EIS）进行生物电极的逐步修饰。差示脉冲伏安法（DPV）研究显示，在最佳条件下，使用亚甲基蓝（MB）作为氧化还原指示剂，目标DNA分析的线性响应范围为10-17至10-9 M，检测限高达3.16？10-18M。另外，所制备的生物传感器提供了区分相似靶DNA序列的主要能力。最终，所提出的生物传感器通过真实样本进行了调查，并显示出出色的性能。因此，开发的ssDNA / Fe3O4 NP / CGO / GCE有望成为实际患者样品分析的候选者。

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