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首页> 外文期刊>International Journal of Environmental Research and Public Health >Seasonal Distribution and Genotyping of Antibiotic Resistant Strains of Listeria Innocua Isolated from A River Basin Categorized by ERIC-PCR
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Seasonal Distribution and Genotyping of Antibiotic Resistant Strains of Listeria Innocua Isolated from A River Basin Categorized by ERIC-PCR

机译：ERIC-PCR分类的流域分离的伊斯特氏菌抗生素耐药菌株的季节性分布和基因分型

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Listeria innocua retains many conserved homologous domains with Listeria monocytogenes , which is a food-borne and water-borne diarrhea-causing bacterium. Studies of antimicrobial resistance in L. innocua showed that this microbe is more prone to acquire resistance than other bacteria in the genus Listeria. However, little is known about the seasonal population distribution and antimicrobial resistance patterns of L. innocua in natural water environments. The aims of the study were: (1) to investigate the occurrence of L. innocua isolates in a subtropical watershed and reconstruct the population structure and (2) to analyze the antibacterial resistance patterns of the identified L. innocua isolates according to ERIC type. A total of 288 water samples was collected from the Puzi River basin (23°28’ N, 120°13’ E) between March 2014 and March 2015, and 36 L. innocua isolates were recovered from 15 positive water samples. With regard to seasonal variation, L. innocua was only detected in the spring and summer. Eighteen enterobacterial repetitive intergenic consensus (ERIC)-PCR types were identified, and two genogroups with four subgroups were reconstructed in a minimum spanning tree. Isolates from different sampling areas that were located near each other were genetically different. All L. innocua isolates (including 41.7% of the multidrug-resistant (MDR) isolates) were resistant to oxacillin and showed high minimum inhibitory concentrations of tetracycline. These findings demonstrate the seasonal variations and differing geographical distributions of L. innocua in this subtropical water environment, as well as the existence of strong population structures and MDR and antimicrobial resistance patterns. Phylogenetic analysis based on ERIC-type showed that the Cluster A isolates were resistant to more antibiotics, and two types, ERIC8 and ERIC15 were multidrug resistant. The more commonly detected types, such as ERIC1 and ERIC12, were also more likely to be resistant to two or more antibiotics. Close monitoring of drug resistance in environmental L. innocua is warranted due to its potential for transferring antimicrobial resistance determinants to pathogenic Listeria.
机译：无病李斯特菌与单核细胞增生性李斯特菌保留许多保守的同源结构域，后者是一种食源性和水源性腹泻细菌。对无毒李斯特菌的抗药性研究表明，与李斯特菌属中的其他细菌相比，这种微生物更容易获得抗药性。然而，人们对自然水环境中无害李斯特菌的季节性种群分布和抗药性模式知之甚少。该研究的目的是：（1）研究亚热带流域中无害李斯特菌的发生并重建种群结构;（2）根据ERIC类型分析鉴定出的无害李斯特菌的抗菌模式。 2014年3月至2015年3月期间，从普子河流域（北纬23°28′，北纬120°13′）收集了288个水样，从15个阳性水样中回收了36株无毒李子。关于季节变化，仅在春季和夏季才检测到无害利什曼原虫。鉴定了十八种肠细菌重复基因间共有（ERIC）-PCR类型，并在最小生成树中重建了具有四个亚组的两个基因组。来自彼此靠近的不同采样区域的分离株在遗传上是不同的。所有无毒李斯特菌分离株（包括41.7％的耐多药（MDR）分离株）均对奥沙西林具有抗性，并显示出最低的四环素抑制浓度。这些发现表明，在这种亚热带水环境中，无害乳杆菌的季节变化和不同的地理分布，以及强大的种群结构，耐多药和耐药性模式的存在。基于ERIC类型的系统进化分析表明，簇A分离株对更多的抗生素具有抗性，而两种类型的ERIC8和ERIC15具有多药耐药性。更常见的检测类型，例如ERIC1和ERIC12，也更有可能对两种或更多种抗生素产生抗药性。由于其可以将抗菌素耐药性决定因素转移到病原性李斯特菌中，因此有必要对环境中的无毒李斯特菌进行严格的耐药性监测。

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