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首页> 外文期刊>International Journal of Phytopharmacy >Endophytic fungi isolation and identification from Memecylon species of Western Ghats, India
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Endophytic fungi isolation and identification from Memecylon species of Western Ghats, India

机译：印度西高止山脉Memecylon物种的内生真菌分离与鉴定

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Endophytic fungi have been documented as sources for novel secondary metabolites with useful medicinal properties. Interest in fungal endophytes is mainly due to their chemical diversity. These signify a virtually untapped source of chemical reservoir that finds applications in agriculture and therapeutics. Sampling and characterization of fungal endophyte diversity is an emerging challenge, which leads to the discovery of new species producing new compounds and a better understanding of their role in ecosystems. In the present study fungal endophytes were isolated from surface sterilized leaf and stem segments of five Memecylon species such as M. umbellatum, M. edule , M. talbotianum, M. malabaricum and M. wightii . A total of 156 isolates of endophytic fungi were obtained from 2000 tissue segments of five Memecylon species being investigated. Of the 156, endophytic isolates recovered, 86 sporulated and belonged to 10 genera, Alternaria (12.2%), Pleosporales (6.4%), Stagonosporopsis (3.8%), Cladosporium (8.2%), Fusarium (7.3%), Aspergillus (12.2%), Pestalopsis (7.5%), Collectotrichum (21.6%), Phoma (4.4%) and fungal mycelia (19.1), which are identified based on morphological characteristics and the dominant endophytes such as C. gleosporiades , Pleosporales sp., Stagonosporopsis cucurbitacearum and other fungal endophytes are further confirmed based on PCR amplification and sequencing of 5.8srRNA gene region and their accessions are obtained KT375578, KT375576.1, KT375577.1 and KT375579.1. The study provides the first report on the isolation and identification of endophytic fungi from Memecylon species.
机译：内生真菌已被记录为具有有用药用特性的新型次生代谢产物的来源。对真菌内生菌的兴趣主要是由于它们的化学多样性。这些标志着实际上尚未开发的化学储藏源，可在农业和治疗领域找到应用。真菌内生菌多样性的采样和表征是一个新兴的挑战，这导致发现了产生新化合物的新物种，并对它们在生态系统中的作用有了更好的了解。在本研究中，从5种Memecylon物种的表面灭菌的叶和茎节中分离了真菌内生菌，如Me。umbellatum，M。edule，M。talbotianum，M。malabaricum和M. wightii。从五个正在研究的Memecylon物种的2000个组织片段中获得了共156种内生真菌分离株。在156个内生分离物中，有86个孢子形成并属于10个属，链霉菌（12.2％），胸膜肺炎（6.4％），链球菌（3.8％），枝孢菌（8.2％），镰刀菌（7.3％），曲霉（12.2％） ），Pestalopsis（7.5％），Collectotrichum（21.6％），Phoma（4.4％）和真菌菌丝体（19.1），它们是根据形态特征和优势内生菌如C. gleosporiades，Pleosporales sp。，Stagonosporopsis cucurbitacearum和根据5.8srRNA基因区域的PCR扩增和测序，进一步确认了其他真菌内生菌，并获得了KT375578，KT375576​​.1，KT375577.1和KT375579.1。该研究提供了关于从Memecylon物种中分离和鉴定内生真菌的第一份报告。

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