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首页> 外文期刊>International Journal of Phytopharmacy >SIMULTANEOUS ESTIAMATION OF DROTAVERINE HCl AND NIMESULIDE IN PHARMACEUTICALS BY HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHIC METHOD
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SIMULTANEOUS ESTIAMATION OF DROTAVERINE HCl AND NIMESULIDE IN PHARMACEUTICALS BY HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHIC METHOD

机译：高效薄层色谱法同时测定药品中的金刚烷胺盐酸盐和亚砜

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摘要

A simple, rapid, precise and accurate high-performance thin-layer chromatographic (HPTLC) method was developed and validated for simultaneous determination of Drotaverine hydrochloride (DRO) and Nimesulide (NIM) in pharmaceutical preparations. Separation was achieved on a Merck HPTLC plates (0.2 mm thickness) precoated with 60 F 254 silica gel on aluminum sheet as the stationary phase using cyclohexane: methanol: ethyl acetate (5:2.:3v/v/v), as the mobile phase. Densitometric quantification was performed at l = 295 nm by reflectance scanning. The R F values of DRO and NIM were obtained 0.41 and 0.62 respectively. The linearity of proposed method was investigated in the range of 0.1 to 0.6 mg/spot and 0.2 to 0.7 mg/spot for DRO and NIM respectively.. The percentage recoveries for DRO and NIM were 99.91 % and 100.19 % by area and 99.63 % and 99.96 % by height, respectively. The developed method was suitably validated for precision, accuracy, specificity and ruggedness.
机译：开发了一种简单，快速，精确和准确的高性能薄层色谱（HPTLC）方法，并验证了该方法可同时测定药物制剂中的盐酸屈他维林（DRO）和尼美舒利（NIM）。使用预先在铝板上涂有60 F 254硅胶的默克HPTLC板（0.2毫米厚）作为固定相进行分离，使用环己烷：甲醇：乙酸乙酯（5：2.：3v / v / v）作为流动相相。通过反射扫描在l ＝ 295nm处进行光密度定量。 DRO和NIM的R F值分别为0.41和0.62。对DRO和NIM分别在0.1至0.6 mg / s和0.2至0.7 mg / s的范围内进行了线性研究。DRO和NIM的回收率分别为99.91％和100.19％（面积）和99.63％和高度分别为99.96％。所开发的方法已针对准确性，准确性，特异性和耐用性进行了适当验证。

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