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首页> 外文期刊>International Journal of Swarm Intelligence and Evolutionary Computation >Combination of PLA Micro-fibers and PCL-Gelatin Nano-fibers for Development of Bone Tissue Engineering Scaffolds
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Combination of PLA Micro-fibers and PCL-Gelatin Nano-fibers for Development of Bone Tissue Engineering Scaffolds

机译：PLA超细纤维和PCL-明胶纳米纤维的组合用于骨组织工程支架的开发

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In tissue engineering, biodegradable porous scaffolds have been employed to replace damaged tissues. These scaffolds are fabricated by conventional techniques such as fiber-bonding, solvent casting, particulate leaching and advanced ones like selective laser sintering, 3-dimentional printing and fused deposition modeling. The main issues limiting conventional techniques are inability to fabricate scaffolds with a highly interconnected porous structure and favorably regular construction with reproducible morphology. Hence researchers go towards advanced techniques with more flexibility. In this study, FDM and electrospinning (ES) techniques have been applied in order to develop multi-layered scaffolds consisting of micro- and nano-fibers for bone tissue engineering applications. While micro- fibrous layers were fabricated via FDM process, nano-fibrous layers were developed using ES technique. Although FDM technique has unique features, its fiber size limits to micro sizes. One of the trends that are useful for eliminating this issue is getting benefit of adding nano-fibers to scaffold's construction. These nano-fibers not only reduce total pore size of the scaffold, but also could improve cell functions. While poly (lactic-acid) (PLA) was used for FDM process, a mixture of poly (caprolactone) (PCL) and gelatin (PCL-gelatin) were applied for electrospinning process to develop interconnected pores with appropriate mechanical properties and degradation rate. The multi-layered scaffolds were examined using electron microscopy (SEM) and their mechanical properties were evaluated. The porosity of the scaffolds was about 40% and results also demonstrated that nano-fibers with size of 200 nm in diameter had good adhesion to micro-fibers and may provide better cell attachment and proliferation.
机译：在组织工程中，可生物降解的多孔支架已被用于替换受损的组织。这些支架是通过常规技术（如纤维粘合，溶剂浇铸，颗粒浸出）和先进技术（如选择性激光烧结，3维印刷和熔融沉积建模）制造的。限制常规技术的主要问题是无法制造具有高度互连的多孔结构的支架，并且不能以可再现的形态良好地进行规则构造。因此，研究人员转向具有更高灵活性的先进技术。在这项研究中，已应用FDM和静电纺丝（ES）技术来开发用于骨组织工程应用的由微纤维和纳米纤维组成的多层支架。虽然通过FDM工艺制造了微纤维层，但使用ES技术开发了纳米纤维层。尽管FDM技术具有独特的功能，但其光纤尺寸仅限于微米尺寸。消除此问题有用的趋势之一是受益于在支架结构中添加纳米纤维。这些纳米纤维不仅可以减小支架的总孔径，而且可以改善细胞功能。当将聚乳酸（PLA）用于FDM工艺时，将聚己内酯（PCL）和明胶（PCL-明胶）的混合物用于电纺丝工艺，以形成具有适当机械性能和降解速率的互连孔。使用电子显微镜（SEM）检查了多层支架，并评估了它们的机械性能。支架的孔隙率约为40％，结果还表明，直径为200 nm的纳米纤维对微纤维具有良好的附着力，可以提供更好的细胞附着和增殖。

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