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首页> 外文期刊>International Journal of Pharmacy and Pharmaceutical Sciences >IN-VITRO CYTOTOXICITY ACTIVITY OF SOLANUM NIGRUM EXTRACT AGAINST HELA CELL LINE AND VERO CELL LINE.
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IN-VITRO CYTOTOXICITY ACTIVITY OF SOLANUM NIGRUM EXTRACT AGAINST HELA CELL LINE AND VERO CELL LINE.

机译：龙葵提取物对Hela细胞株和VERO细胞株的体外细胞毒性活性。

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The study was aimed to evaluation of the anticancer activity of the fruits of Solanum Nigrum on the HeLa cell line. The fruits of Solanum Nigrum methanolic extract were tested for its inhibitory effect on HeLa Cell Line. The percentage viability of the cell line was carried out by using Trypan blue dye exclusion method. The cytotoxicity of Solanum Nigrum on HeLa cell was evaluated by the SRB assay and MTT assay. Solanum Nigrum methanolic extract has significant cytotoxicity effect on HeLa Cell Line in concentration range between 10 mg/ml to 0.0196 mg/ml by using SRB assay and study also showed that inhibitory action on HeLa cell line in concentration range between 10 mg/ml to 0.0196 mg/ml by using MTT assay. IC50value and R2value of Solanum Nigrum on HeLa cell and Vero cell were 847.8 and 0.8724, 9088 and 0.1017 respectively by SRB assay. IC50 value and R2value of Solanum Nigrum on HeLa cell was 265.0 and 0.9496 respectively by MTT assay. IC50 value of Solanum Nigrum on Vero cell was 6.862 by MTT assay. R2value of Solanum Nigrum was not found by MTT assay. From the performed assay, methanolic extract of these drug shows greater activity on HeLa cell line and little activity on Vero cell line and that mean Solanum Nigrum can be used as anticancer
机译：该研究旨在评估黑茄果实对HeLa细胞系的抗癌活性。测试了龙葵甲醇提取物的果实对HeLa细胞系的抑制作用。通过使用锥虫蓝染料排除法进行细胞系的存活率百分比。用SRB法和MTT法评价了龙葵对HeLa细胞的细胞毒性。用SRB分析法测定龙葵甲醇提取物对HeLa细胞系的细胞毒性作用，浓度范围为10 mg / ml至0.0196 mg / ml，研究还表明，浓度范围为10 mg / ml至0.0196的HeLa细胞系具有抑制作用通过使用MTT测定法测定mg / ml。通过SRB分析，龙葵对HeLa细胞和Vero细胞的IC 50值和R 2值分别为847.8和0.8724、9088和0.1017。 MTT法检测黑龙葵对HeLa细胞的IC50值和R2值分别为265.0和0.9496。通过MTT分析，龙葵对Vero细胞的IC50值为6.862。通过MTT分析未发现龙葵的R2值。从进行的测定中，这些药物的甲醇提取物对HeLa细胞系表现出更大的活性，而对Vero细胞系表现出很小的活性，这意味着黑茄可以用作抗癌药。

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