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首页> 外文期刊>International Research Journal of Biological Sciences >Quantification of LUPEOL in Excoecaria agallocha Leaf, Stem and Root by HPTLC
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Quantification of LUPEOL in Excoecaria agallocha Leaf, Stem and Root by HPTLC

机译：高效液相色谱法定量测定非洲Exececaria agallocha叶，茎和根中LUPEOL的含量

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An attempt to augment to quantify Lupeol, pharmacologically bioactive triterpenoid in the Excoecaria Agallocha mangrove (methanol extracts of leaf, stem, root parts), has been made. A HPTLC method was developed with the combination of n- Hexane: Ethyl acetate: Methanol (9:1:1 v/v) as solvent system and TLC silica gel 60 F254plates as stationary phase under constant room temperature and relative humidity of 26.1 - 0.4 0C and 76 - 2 % respectively. Detection and quantification were performed by densitometry scanning at 540 nm after derivatization with Anisaldehide Sulfuric Acid Reagent (ASR). This method gave compact spots at RF 0.31 corresponding to Lupeol. As a result, the content of Lupeol in the leaf, stem and root were found 492.42 ng, 704.23 ng and 728.15 ng respectively as well as % amount (w/w) of Lupeol in the leaf, stem and root were found 0.09948, 0.14085 and 0.14568 respectively.
机译：已经尝试增加定量法对露齿草Agallocha红树林（叶，茎，根部分的甲醇提取物）具有药理生物活性的三萜类化合物Lupeol进行定量。在恒定的室温和26.1-0.4的相对湿度下，开发了一种HPTLC方法，该方法结合使用正己烷：乙酸乙酯：甲醇（9：1：1 v / v）和固定相TLC硅胶60 F254板为固定相的溶剂系统0C和76-2％。在用茴香醚乙二酸酐硫酸试剂（ASR）衍生化之后，通过在540 nm处的光密度法扫描进行检测和定量。该方法在RF 0.31处给出了对应于Lupeol的致密斑点。结果，发现叶，茎和根中卢贝醇的含量分别为492.42 ng，704.23 ng和728.15 ng，以及叶，茎和根中卢贝醇的含量（w / w）0.09948、0.14085和0.14568。

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