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首页> 外文期刊>Iranian Journal of Basic Medical Sciences >EVALUATION OF THE EFFECT OF THE 47 KDA PROTEIN ISOLATED FROM AGED GARLIC EXTRACT ON DENDRITIC CELLS
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EVALUATION OF THE EFFECT OF THE 47 KDA PROTEIN ISOLATED FROM AGED GARLIC EXTRACT ON DENDRITIC CELLS

机译：老化大蒜提取物中47 KDA蛋白质对树突状细胞的作用评估

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Objective(s): Garlic (Allium sativum) is known as a potent spice and a medicine with broad therapeutic properties ranging from antibacterial to anticancer, and anticoagulant. One of the major purified garlic protein components is the 47 kDa protein. In this study, the effect of 47 kDa protein extracted from aged garlic (AGE) was evaluated on mouse dendritic cell (DC) maturation in vitro. Materials and Methods: Forty seven kDa protein was purified from AGE by ammonium sulfate precipitation and gel filtration. SDSPAGE was used to determine the molecular weight and purity of the isolated protein. DCs were purified from spleen of BALB/c mice by Nycodenz centrifugation and their adhesiveness to the plastic dish. The 47 kDa protein isolated from AGE was added to DCs medium during the overnight culture and the expression of DC surface markers was assessed via flowcytometry. Results: The 47 kDa protein-treated DCs lowered the expression of DC maturation markers including: CD40, CD86 and MHC-II in comparison with non-treated DCs, (median of 41% versus 47%, 84% versus 91% and 83% versus 90%, respectively) but we observed no statistical difference between the two groups. Conclusion: Upon treatment with DCs with 47 kDa protein, DCs down regulated the expression of costimulatory and MHC-II surface molecules, which is similar to tolerogenic DC phenotype. According to the results of the present study, we found that 47 kDa protein purified from AGE can be considered as a potential candidate to generate tolerogenic DCs in vitro.
机译：目标：大蒜（大蒜）被认为是一种有效的香料，是一种具有广泛治疗特性的药物，其抗菌，抗癌和抗凝作用广泛。 47 kDa是主要的纯化大蒜蛋白成分之一。在这项研究中，评估了从衰老大蒜（AGE）中提取的47 kDa蛋白对小鼠树突状细胞（DC）体外成熟的影响。材料和方法：通过硫酸铵沉淀和凝胶过滤从AGE中纯化出47 kDa蛋白。 SDSPAGE用于确定分离的蛋白质的分子量和纯度。通过Nycodenz离心从BALB / c小鼠的脾脏中纯化DC，并将其与塑料皿粘附。在过夜培养期间，将从AGE分离的47kDa蛋白添加到DCs培养基中，并通过流式细胞术评估DC表面标志物的表达。结果：与未处理的DC相比，经47 kDa蛋白处理的DC降低了DC成熟标记的表达，包括：CD40，CD86和MHC-II（中位数分别为41％对47％，84％对91％和83％）分别为90％和90％），但我们观察到两组之间没有统计学差异。结论：用47 kDa蛋白的DC处理DC后，DC会下调共刺激分子和MHC-II表面分子的表达，这与致耐受DC表型相似。根据本研究的结果，我们发现从AGE纯化的47 kDa蛋白可以被认为是在体外产生耐受DC的潜在候选者。

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