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首页> 外文期刊>Cellular immunology >Evaluation of the immunomodulatory effect of the 14 kDa protein isolated from aged garlic extract on dendritic cells.
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Evaluation of the immunomodulatory effect of the 14 kDa protein isolated from aged garlic extract on dendritic cells.

机译：评估从老化大蒜提取物中分离的14 kDa蛋白对树突状细胞的免疫调节作用。

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摘要

Garlic is used all over the world for treatment of different diseases. A wide range of biological activities of garlic has been verified in vitro and in vivo. One of major proteins of garlic which has been isolated and purified is the 14 kDa protein. This protein has been shown to have immunomodulatory effects. In this study, the effect of the 14 kDa protein isolated from aged garlic extract (AGE) was investigated on maturation and immunomodulatory activity of dendritic cells (DC). Proteins were purified from AGE by biochemical method; the semi-purified 14 kDa protein was run on gel filtration Sephadex G50 and its purity was checked by SDS-PAGE. DC were isolated from spleen of BALB/c mice by Nycodenz centrifugation and their adhesiveness to plastic dish. 14 kDa protein from AGE was added to overnight culture of DC medium and the expression percentage of CD40, CD86, and MHC-II was evaluated by flowcytometric analysis. Also, proliferation of T-cells was measured by allogenic mixed lymphocyte reaction (MLR) test. The purified 14 kDa protein isolated from AGE increased the expression of CD40 molecule on DC, but it did not influence CD86 and MHCII molecules. Furthermore, no significant differences were noticed in the pulsed-DC with 14 kDa protein and non-pulsed DC on the MLR.
机译：大蒜在世界各地用于治疗各种疾病。大蒜的多种生物活性已在体外和体内得到证实。已分离和纯化的大蒜的主要蛋白质之一是14 kDa蛋白质。该蛋白质已显示具有免疫调节作用。在这项研究中，研究了从陈年大蒜提取物（AGE）中分离的14 kDa蛋白对树突状细胞（DC）成熟和免疫调节活性的影响。通过生化方法从AGE中纯化蛋白质;将半纯化的14 kDa蛋白在凝胶过滤Sephadex G50上运行，并通过SDS-PAGE检查其纯度。通过Nycodenz离心从BALB / c小鼠的脾脏中分离DC，并将其与塑料皿粘附。将来自AGE的14kDa蛋白添加到DC培养基的过夜培养物中，并通过流式细胞术分析评估CD40，CD86和MHC-II的表达百分比。另外，通过同种异体混合淋巴细胞反应（MLR）测试来测量T细胞的增殖。从AGE中分离出的纯化的14 kDa蛋白增加了DC上CD40分子的表达，但不影响CD86和MHCII分子。此外，在MLR上的具有14 kDa蛋白的脉冲DC和非脉冲DC中，未发现明显差异。

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