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首页> 外文期刊>Egyptian Journal of Medical Human Genetics >Association assessment of Interleukine-10 gene polymorphism and its expression status with susceptibility to coronary artery disease in Iran
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Association assessment of Interleukine-10 gene polymorphism and its expression status with susceptibility to coronary artery disease in Iran

机译：伊朗 Interleukine-10 基因多态性及其表达状况与冠心病易感性的关联性评估

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Background The cytokines are potent inflammatory factors that regulate each stage of atherosclerosis leading to the disease development. Interleukin-10 (IL-10) as an anti-inflammatory cytokine can develop atherosclerosis by inhibiting the synthesis of metalloproteinase. Moreover, IL-10 promotes the plaque stability by preserving the extracellular matrix and fibrous cap. Aim We evaluated the association of the two IL-10 promoter gene polymorphisms with susceptibility to coronary artery disease (CAD) in Iranian population. Subjects and methods We used the Sequence Specific Primer-Polymerase Chain Reaction method to determine genotypes. We also studied mRNA expression of the IL-10 gene in Iranian CAD patients using quantitative real-time PCR. Results There was a significant association between IL-10 (?819) T allele and IL-10 (?819) T/T genotype, and CAD (p=0.041 and p=0.042 respectively). There also was a significant association between IL-10 (?1082) G allele and IL-10 (?1082) G/G genotype, and CAD (p=0.017 and p=0.020 respectively). Genotype T/T of IL-10 (?819) polymorphism significantly associated with the two vessel disease type (p=0.017). In addition, there was a significant association between IL-10 (?1082) G/G genotype and the three vessel disease type (p=0.009). IL-10 mRNA expression was significantly decreased 3.36-fold in samples with IL-10 (?819) polymorphism and 1.98-fold in individuals with IL-10 (?1082) polymorphism. Conclusions Our results suggest that IL-10 gene promoter polymorphisms may influence both coronary artery disease risks and severity in Iranian patients.
机译：背景技术细胞因子是有效的炎症因子，可调节导致疾病发展的动脉粥样硬化的各个阶段。白介素10（IL-10）作为一种抗炎细胞因子，可通过抑制金属蛋白酶的合成而发展为动脉粥样硬化。此外，IL-10通过保留细胞外基质和纤维帽来促进噬菌斑稳定性。目的我们评估了伊朗人群中两个IL-10启动子基因多态性与对冠心病（CAD）的敏感性之间的关系。受试者和方法我们使用序列特异性引物-聚合酶链反应方法确定基因型。我们还使用定量实时PCR研究了伊朗CAD患者中IL-10基因的mRNA表达。结果IL-10（？819）T等位基因和IL-10（？819）T / T基因型与CAD之间存在显着相关性（分别为p = 0.041和p = 0.042）。 IL-10（？1082）G等位基因和IL-10（？1082）G / G基因型与CAD之间也存在显着关联（分别为p = 0.017和p = 0.020）。 IL-10（？819）多态性的基因型T / T与两种血管疾病类型显着相关（p = 0.017）。此外，IL-10（？1082）G / G基因型与三支血管疾病类型之间存在显着关联（p = 0.009）。在具有IL-10（？819）多态性的样本中，IL-10 mRNA的表达显着降低了3.36倍，而在具有IL-10（？1082）多态性的个体中，其表达降低了1.98倍。结论我们的结果表明IL-10基因启动子多态性可能会影响伊朗患者的冠状动脉疾病风险和严重程度。

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