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首页> 外文期刊>Iranian Journal of Reproductive Medicine >THE IN-VITRO EFFECTS OF NICOTINE, COTININE AND LEPTIN ON SPERM PARAMETERS ANALYZED BY CASA SYSTEM
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THE IN-VITRO EFFECTS OF NICOTINE, COTININE AND LEPTIN ON SPERM PARAMETERS ANALYZED BY CASA SYSTEM

机译：烟碱，可替宁和瘦素对CASA系统分析精子参数的体外影响

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Backgrand: Cotinine (COT) is a major degradation product of nicotine (NIC). The participation of leptin in female reproduction is well-established, but any role in male reproductive function is at the best tenuous. Objective: The aim of this study was to evaluate the in-vitro effects of nicotine, cotinine and leptin on sperm parameters in normal semen of non-smokers fertile men.Materials and Methods: Ten healthy nonsmokers aged 25-40 years old were devided into 7 groups, Thier semens were divided into 7 aliquots. (A) was layered with basal solution 70 ng/ml NIC, (B) 35 mg/ml NIC, (C) 300 ng/ml COT, (D) 200 mg/ml COT, (E) 30 ng/ml leptin, (F) 300 ng/ml leptin respectively and (G) was layered with mHTF. After migration, the samples were examined at time 0, +1, +2, +4, +8, and + 24 h of incubation. Results: These findings were obtained: sperm count: 75.66?±66.25x10 6 /ml, forward motility: 75.55?±14.80%, progress: 33.66?±13.01, VSL: 51.58?±6.99 mm/s, VCL: 103.33?±14.52 mm/s, ALH: 4.33?±0.77 mm, BCF: 25.60?±2.97 HZ, STR: 79.33?±8.04 %, LIN: 52.55?±10.52 %, ELO: 74.22 ?±12.76 % and ARE: 3.04?±1.50 u/sq. The parameters were similar before 8 hr and were being decreased after that. Conclusion: According to the results. nicotin and cotinin have negative effects on the sperm parameters but despite the positive effect of leptin, there is no correlation between leptin concentration in semen and its physical characteristics.
机译：背景：可替宁（COT）是尼古丁（NIC）的主要降解产物。瘦蛋白在女性生殖中的参与是公认的，但是在男性生殖功能中的任何作用最好都是微不足道的。目的：本研究的目的是评估尼古丁，可替宁和瘦素对非吸烟可育男性正常精液中精子参数的体外作用。材料与方法：分为十个年龄在25-40岁之间的健康非吸烟者。 7个小组，他们的精液被分成7等分。 （A）用基础溶液70 ng / ml NIC分层，（B）35 mg / ml NIC，（C）300 ng / ml COT，（D）200 mg / ml COT，（E）30 ng / ml瘦素， （F）分别为300 ng / ml瘦蛋白，（G）用mHTF分层。迁移后，在孵育时间0，+ 1，+ 2，+ 4，+ 8和+24小时检查样品。结果：获得了这些发现：精子数：75.66±66.25x10 6 / ml，向前运动：75.55±14.80％，进展：33.66±13.01，VSL：51.58±6.99 mm / s，VCL：103.33± 14.52 mm / s，ALH：4.33±0.77 mm，BCF：25.60±±2.97 HZ，STR：79.33±±8.04％，LIN：52.55±±10.52％，ELO：74.22±±12.76％，ARE：3.04± 1.50 u /平方这些参数在8小时之前是相似的，此后逐渐降低。结论：根据结果。烟碱和可替宁对精子参数有负面影响，但尽管瘦素具有积极作用，但精液中瘦素浓度与其物理特性之间没有相关性。

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