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首页> 外文期刊>Iranian Journal of Reproductive Medicine >THE EFFECTS OF FOLLICULAR FLUID ON HUMAN X, Y BEARING SPERM RATIO BY FLUORESCENT IN SITU HYBRIDIZATION (FISH)
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THE EFFECTS OF FOLLICULAR FLUID ON HUMAN X, Y BEARING SPERM RATIO BY FLUORESCENT IN SITU HYBRIDIZATION (FISH)

机译：荧光原位杂交对人X，Y人精子比的影响

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Background: Some evidences showed that the secretion of uterine tube, vagina andfollicular fluid (FF) affects X and Y-chromosome populations. Sperm selection with X or Y chromosome can added to oocyte for gender desired. The isolation of X, Y sperm have done and all efforts in this field are done to make culture media similar to in vivo condition. The objective of this study was to find if the FF can influence the ratio of X or Y chromosomes, therefore we added human FF to culture media to separate X and Y sperms.Materials and Methods: Normal semen sample from 36 healthy men were selected. Then the samples were divided into control and experimental groups: control group sperms have been incubated with conventional culture media (Ham's F-10) and experimental group with conventional culture media + 10% human FF. For sperm isolation, swim up technique was used. After 24 hours of incubation, slides smear were prepared. Then, we used the Fluorescent in Situ Hybridization (FISH) method to evaluate the effect of follicular fluid on the population ratio of X and Y containing sperms.Results: Although the incubation of sperm in FF and Ham's F-10 increased Y sperm (59.44 % in control and 61.42% in experimental groups) in comparison with X sperm (40.56% in control and 38.5 in experimental groups) significantly (p0.05), but the Y (or X) bearing sperm did not significantly change in experimental group in comparison with Y (or X) bearing sperm in control group.Conclusion: This study showed that using the swim up method for collecting sperms and adding FF to culture media can improve some sperm parameters, but did not has significant effects on population of X and Y sperm.
机译：背景：一些证据表明子宫管，阴道和卵泡液（FF）的分泌会影响X和Y染色体群体。可以将具有X或Y染色体的精子选择添加到卵母细胞中以获得所需的性别。 X，Y精子的分离已经完成，并且在该领域已尽一切努力使培养基类似于体内条件。这项研究的目的是发现FF是否可以影响X或Y染色体的比率，因此我们将人类FF添加到培养基中以分离X和Y精子。材料与方法：选择36名健康男性的正常精液样本。然后将样品分为对照组和实验组：对照组的精子与常规培养基（Ham's F-10）一起孵育，实验组与常规培养基+ 10％人FF孵育。对于精子隔离，使用游泳技术。温育24小时后，制备载玻片涂片。然后，我们使用荧光原位杂交（FISH）方法评估了卵泡液对含X和Y的精子的种群比率的影响。结果：尽管在FF和Ham's F-10中孵化的精子增加了Y精子（59.44与X精子比较（对照组为40.56％，实验组为38.5），对照组中的％（实验组中为61.42％）（p <0.05），而在实验组中，带有Y（或X）的精子在实验组中没有显着变化。结论：本研究表明，采用游动法收集精子并向培养基中添加FF可以改善某些精子参数，但对X和X的种群没有显着影响。 Y精子。

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