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首页> 外文期刊>Iranian Journal of Reproductive Medicine >DOES WOMENa€?S AGE INFLUENCE ZONA PELLUCIDA BIREFRINGENCE OF METAPHASE ?? OOCYTES IN IN-VITRO MATURATION PROGRAM?
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DOES WOMENa€?S AGE INFLUENCE ZONA PELLUCIDA BIREFRINGENCE OF METAPHASE ?? OOCYTES IN IN-VITRO MATURATION PROGRAM?

机译：妇女的年龄是否影响了甲胎蛋白双胞胎的双折射？卵在体外成熟程序中？

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Background: In vitro maturation (IVM) is a promising treatment option for certain infertile women. Nowadays, with the aid of PolScope, it has become possible to evaluate zona pellucida (ZP) characteristics as a parameter of oocyte quality. Moreover, quality of oocytes can be influenced by many factors, such as patienta€?s age. The PolScope system is a non-invasive technique to assess birefringent structures such as the meiotic spindle and ZP in living oocytes.Objective: The aim was to determine the influence of the woman's age on ZP birefringence, a sign of oocyte quality, and morphology of in-vitro matured human oocytes using non-invasive polarized light (PolScope) microscopy.Materials and Methods: ZP birefringence and morphology were determined in 105 retrieved oocytes from 58 women undergoing ICSI in two age groups (?30 years and 30 years). The immature oocytes were selected and after IVM, the quality of metaphase ?? (MII) oocytes was assessed. The oocytes abnormalities were classified as intracytoplasmic and extracytoplasmic abnormalities.Results: Oocyte maturation rates were significantly reduced in ?30 yeara€?s women (56%) in comparison with other age group (80.7%). In addition, the ZP birefringence was significantly higher in MII oocytes in the younger group compared with the older group (76.2% vs. 38.1%; p=0.00). Following morphologic assessment, the rates of oocytes with extracytoplasmic (p=0.02) and both abnormalities (extra- and intracytoplasmic) (p=0.01) were higher in aged versus the younger women.Conclusion: There was a positive relationship between advanced maternal age with decreased ZP birefringence and oocyte morphological quality in in-vitro matured human oocytes.
机译：背景：体外成熟（IVM）是某些不育女性的有前途的治疗选择。如今，借助PolScope，可以评估透明带（ZP）特性作为卵母细胞质量的参数。此外，卵母细胞的质量会受到许多因素的影响，例如患者的年龄。 PolScope系统是一种非侵入性技术，用于评估活卵母细胞中的双折射结构（如减数分裂纺锤体和ZP）。目的：确定女性年龄对ZP双折射，卵母细胞质量的征兆和形态的影响材料和方法：在两个年龄段（≥30岁和<30岁）的58位接受ICSI的女性中，从105例回收的卵母细胞中测定了ZP双折射和形态。选择未成熟的卵母细胞，经过IVM后，中期的质量（MII）卵母细胞进行了评估。结果：30岁女性（56％）的卵母细胞成熟率比其他年龄组（80.7％）明显降低。此外，年轻组的MII卵母细胞的ZP双折射显着高于老年组（分别为76.2％和38.1％; p = 0.00）。经过形态学评估，老年女性的卵母细胞中胞浆外（p = 0.02）和两种异常（胞外和胞浆内）（p = 0.01）的发生率均高于年轻女性。体外成熟的人卵母细胞降低ZP双折射和卵母细胞形态质量。

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