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首页> 外文期刊>Iranian red crescent medical journal >Hypnotic Effect of Ocimum basilicum on Pentobarbital-Induced Sleep in Mice
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Hypnotic Effect of Ocimum basilicum on Pentobarbital-Induced Sleep in Mice

机译：罗勒皮对戊巴比妥诱发的小鼠睡眠的催眠作用

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摘要

Sleep disorders are accompanied by several complications, and currently used soporific drugs can induce unwanted effects such as psychomotor impairment, tolerance, amnesia, and rebound insomnia. Objectives: The present study was carried out to investigate if Ocimum basilicum has a sleep-prolonging effect. Materials and Methods: This work was an experimental study on 72 mice which were randomly divided into 9 groups: saline (control); diazepam (3 mg/kg, positive control); hydro-alcoholic extract (HAE) of Ocimum basilicum (25, 50, or 100 mg/kg); ethyl acetate fraction (EAF, 50 mg/kg); n-butanol fraction (NBF, 50 mg/kg); water fraction (WF, 50 mg/kg); and saline containing 10% DMSO (vehicle for EAF and NBF). All the test compounds were injected intraperitoneally (IP) 30 minutes before pentobarbital administration (30 mg/kg). Duration and latency of pentobarbital-induced sleep were recorded. Also, LD50 of HAE was determined and the cytotoxicity of HAE was tested on neural and fibroblast cells using the MTT assay. Results: HAE increased the duration of pentobarbital-induced sleep at doses of 25, 50, and 100 mg/kg (P < 0.001). The hypnotic effect of HAE was comparable to that induced by diazepam. Similarly, WF, EAF, and NBF at 50 mg/kg could increase sleep duration. The sleep latency was decreased by HAE (P < 0.01 - P < 0.001) and NBF (P < 0.001), but not by WF and EAF. The LD50 value for HAE was found to be 2.4 g/kg. HAE had no effect on the viability of neuronal PC12 cells and L929 fibroblast cells. Conclusions: The present data demonstrated that Ocimum basilicum potentiates sleeping behaviors without any cytotoxicity. The main component (s) responsible for the hypnotic effects of this plant is most likely a non-polar agent (s) which is found in NBF. Isolation of the active constituents may yield a novel sedative drug.
机译：睡眠障碍会伴有多种并发症，目前使用的多孔药物会诱发不良反应，例如精神运动障碍，耐受性，健忘症和反弹性失眠。目的：进行本研究以调查罗勒罗非鱼是否具有延长睡眠的作用。材料与方法：这项工作是对72只小鼠进行的实验研究，将其随机分为9组：生理盐水（对照组）和生理盐水（对照组）。安定（3 mg / kg，阳性对照）;罗勒罗汉果（25、50或100 mg / kg）的水醇提取物（HAE）;乙酸乙酯级分（EAF，50 mg / kg）;正丁醇级分（NBF，50 mg / kg）;水分数（WF，50 mg / kg）;以及含10％DMSO的盐水（用于EAF和NBF的车辆）。在戊巴比妥给药前30分钟腹膜内（IP）注射所有受试化合物（30 mg / kg）。记录戊巴比妥诱发的睡眠的持续时间和潜伏期。另外，使用MTT测定法测定HAE的LD 50，并在神经和成纤维细胞上测试HAE的细胞毒性。结果：HAE以25、50和100 mg / kg的剂量增加了戊巴比妥诱发的睡眠时间（P <0.001）。 HAE的催眠作用与地西epa诱导的催眠作用相当。同样，WF，EAF和NBF为50 mg / kg可以增加睡眠时间。 HAE（P <0.01-P <0.001）和NBF（P <0.001）减少了睡眠潜伏期，而WF和EAF则没有。发现HAE的LD 50值为2.4g / kg。 HAE对神经元PC12细胞和L929成纤维细胞的活力没有影响。结论：目前的数据表明罗勒罗勒增强了睡眠行为而没有任何细胞毒性。负责该植物催眠作用的主要成分很可能是一种在NBF中发现的非极性物质。活性成分的分离可以产生新的镇静药。

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