首页> 外文期刊>Egyptian Dermatology Online Journal >Phenotypic characterization of inflammatory cellular infiltrate in lesional skin from patients with pityriasis rosea
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Phenotypic characterization of inflammatory cellular infiltrate in lesional skin from patients with pityriasis rosea

机译:玫瑰糠疹患者病变皮肤炎症细胞浸润的表型特征

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BackgroundPityriasis rosea (PR) is an acute, self-limiting papulosquamous skin disease. T-helper lymphocytes play an important role in the pathogenesis of PR. However, the role of cytotoxic T-lymphocytes is poorly understood.ObjectiveTo investigate the immune profile of mixed inflammatory cell infiltrate in skin lesions with PR.MethodsThe study included 10 biopsy specimens from lesional skin with clinical diagnosis of PR . Ten biopsy specimens from normal skin served as a control. Monoclonal antibodies were used for targeting antigenic epitopes on several cell types including CD3 (for T-cells), CD20 (for B-cells), CD68 (for macrophage/dendritic cells), and Granzyme B (Gr B) (for active cytotoxic T-cells).ResultsLevels of CD3 +ve, CD68 and Gr B were significantly higher in lesional skin as compared to normal skin (p0.05). CD3 +ve cells was the predominant cell population in lesional skin.ConclusionThe finding of significantly increased numbers of CD3 +ve cells in skin lesions with PR indicates an important role of cell mediated immunity in the pathogenesis of the disease. A significantly increased number of Gr B +ve cells in all lesions suggested that a fraction of CD3 +ve cells have an active cytotoxic potential.
机译:背景玫瑰糠疹(PR)是一种急性自限性丘疹性皮肤病。 T辅助淋巴细胞在PR的发病机理中起重要作用。然而,对细胞毒性T淋巴细胞的作用了解甚少。目的探讨PR混合性炎症细胞浸润在皮肤病灶中的免疫特性。方法:本研究包括10例病灶皮肤活检标本,临床诊断为PR。正常皮肤的十个活检标本作为对照。单克隆抗体用于靶向几种细胞类型上的抗原表位,包括CD3(对于T细胞),CD20(对于B细胞),CD68(对于巨噬细胞/树突状细胞)和粒酶B(Gr B)(对于活性细胞毒性T)结果与正常皮肤相比,病变皮肤中CD3 + ve,CD68和Gr B的水平显着更高(p <0.05)。 CD3 + ve细胞是病变皮肤中的主要细胞群。结论在PR皮肤病变中CD3 + ve细胞数量的明显增加表明细胞介导的免疫在该病的发病机理中具有重要作用。在所有病变中,Gr B + ve细胞的数量显着增加,表明一部分CD3 + ve细胞具有活跃的细胞毒性潜能。

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