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首页> 外文期刊>Epigenetics & Chromatin >MacroH2A subtypes contribute antagonistically to the transcriptional regulation of the ribosomal cistron during seasonal acclimatization of the carp fish
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MacroH2A subtypes contribute antagonistically to the transcriptional regulation of the ribosomal cistron during seasonal acclimatization of the carp fish

机译：MacroH2A亚型在鲤鱼的季节适应过程中对核糖体顺反子的转录调控起拮抗作用

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Background Incorporation of histone variants into chromatin is one of the epigenetic mechanisms used for regulation of gene expression. Macro (m)H2A is a histone variant that has two different subtypes in vertebrates: mH2A1 and mH2A2. It is known that mH2A is associated with gene silencing, but recent studies indicate that these mH2A subtypes could contribute more widely to transcriptional regulation. We have previously demonstrated that the gene-reprogramming response mediates adaptation of the carp fish to its environment, and that ribosomal gene transcription is seasonally regulated in carp. However, there have been few studies investigating how epigenetic mechanisms contribute to environmental adaptation and, in particular, to ribosomal cistron regulation. Results In this paper, we report the occurrence of differential incorporation of mH2A subtypes into chromatin during seasonal adaptation in the carp, an event that concurs with opposing transcriptional states. Moreover, we observed that enrichment of mH2A1 in the ribosomal cistron during winter, and conversely, enrichment of mH2A2 during summer. mH2A1 consistently colocalizes with a heterochromatin marker (H3K27me2; histone H3 trimethylated at lysine 27) and mH2A2 with a euchromatin marker (H3K4me3; histone H3 trimethylated at lysine 4). Similar results were found for the L41gene, with enrichment of mH2A in the promoter region. Conclusions We have characterized both mH2A subtypes from carp fish, and evaluated their participation in the regulation of the ribosomal cistron. Our findings indicate that differential incorporation of mH2A subtypes into the ribosome could regulate gene expression during the acclimatization process in carp. Our results reveal differential chromatin incorporation of the mH2A subtypes during the environmental adaptation process, correlating wtih antagonistic transcriptional states in the carp ribosomal cistron.
机译：背景技术将组蛋白变体掺入染色质是用于调节基因表达的表观遗传机制之一。宏（m）H2A是一种组蛋白变异体，在脊椎动物中具有两种不同的亚型：mH2A1和mH2A2。众所周知，mH2A与基因沉默有关，但是最近的研究表明，这些mH2A亚型可以更广泛地参与转录调控。我们以前已经证明，基因重编程反应介导了鲤鱼对环境的适应，并且核糖体基因转录在鲤鱼中受到季节性调节。但是，很少有研究调查表观遗传机制如何促进环境适应，尤其是对核糖体顺反子调节的影响。结果在本文中，我们报道了鲤鱼在季节适应过程中发生了染色质中mH2A亚型差异性掺入的现象，这与相反的转录状态一致。此外，我们观察到冬季核糖体顺反子中mH2A1富集，相反，夏季时mH2A2富集。 mH2A1始终与异染色质标记（H3K27me2;组蛋白H3在赖氨酸27处三甲基化）共存，而mH2A2与常染色质标记（H3K4me3;组蛋白H3在赖氨酸4处三甲基化）共存。对于L41基因，在启动子区域富集了mH2A，发现了相似的结果。结论我们已经鉴定了鲤鱼的两种mH2A亚型，并评估了它们在核糖体顺反子调控中的参与。我们的发现表明，在鲤鱼的驯化过程中，mH2A亚型不同地掺入核糖体可以调节基因表达。我们的结果揭示了在环境适应过程中mH2A亚型的染色质差异性掺入，与鲤鱼核糖体顺反子中的拮抗转录状态相关。

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