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首页> 外文期刊>Eurasian Journal of Analytical Chemistry >Simultaneous Quantitative Determination of Imidazole Antimycotics in Human Urine by using RPLC Technique
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Simultaneous Quantitative Determination of Imidazole Antimycotics in Human Urine by using RPLC Technique

机译：RPLC技术同时定量测定人尿中咪唑类抗真菌药

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Imidazoles are well known heterocyclic compounds having important feature of a variety of medicinal agents. Studies with imidazole containing compounds are great importance in the clinical use of drugs. The presence of imidazole group in the molecules of lanoconazole (LCZ) and butoconazole (BCZ) is very important for its chemical and analytical behavior. Target analytes were efficiently separated with a Pinnacle DB Cyano column (5 ?μm, 4.6 x 250 mm I.D.) and oxiconazole (OCZ) used as an internal standard. In this study, correlation between the retention factors of the solutes and pH of the mobile phase was determined at ten pH values in acetonitrile-water binary mixture. In optimization condition, quantification of these drugs were performed in human urine. The linear responses were observed over a wide concentration range (6.25.10-6-3.13.10-5 mol L-1 for LCZ and 6.32.10-6 - 2.53.10-5 mol L-1 for BCZ) in human urine. The method were validated by determining the limits of detection (LODs, 2.01.10-6 - 5.50.10-7 mol L-1), the limits of quantification (LOQs, 6.08.10-6 - 1.67.10-6 mol L-1), recoveries (99.14%, 99.93%) and intra-day and inter-day relative standard deviations (RSDs, 1%). The data presented in this report demonstrate a rapid, sensitive and accurate method for the simultaneous analysis of LCZ and BCZ in human urine for pharmacokinetic studies.
机译：咪唑是众所周知的具有多种药物重要特征的杂环化合物。用含咪唑的化合物进行的研究在药物的临床使用中非常重要。兰诺康唑（LCZ）和丁康唑（BCZ）分子中咪唑基的存在对其化学和分析行为非常重要。使用Pinnacle DB Cyano色谱柱（5 µm，内径4.6 x 250 mm）和奥克康唑（OCZ）作为内标，可以有效地分离目标分析物。在这项研究中，在乙腈-水二元混合物的十个pH值下确定了溶质的保留因子与流动相的pH之间的相关性。在最佳条件下，在人尿中对这些药物进行了定量。在人类尿液中的宽浓度范围内观察到线性响应（LCZ为6.25.10-6-3.13.10-5 mol L-1，BCZ为6.32.10-6-2.53.10-5 mol L-1） 。通过确定检测限（LODs，2.01.10-6-5.50.10-7 mol L-1），定量限（LOQs，6.08.10-6-1.67.10-6 mol L）验证该方法-1），回收率（99.14％，99.93％）以及日内和日间相对标准偏差（RSDs <1％）。本报告中提供的数据证明了同时分析人尿中LCZ和BCZ的快速，灵敏和准确的方法，用于药代动力学研究。

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