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首页> 外文期刊>European Journal of Histochemistry >Effect of retinoic acid on the nitrergic innervation of meibomian glands in rats
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Effect of retinoic acid on the nitrergic innervation of meibomian glands in rats

机译：维甲酸对大鼠睑板腺硝化神经支配的影响

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The purpose of this study was to investigate the effect of prenatal administration of retinoic acid (RA) on the development of nicotinamide adenine dinucleotide diaphorase (NADPH-d) positive structures in the rat Meibomian glands. One mg/kg of RA was applied to pregnant Wistar rats intraperitonaelly during the gestational period in each of the 12th-14th embryonic days (totally 3 mg/kg). Sections of the central upper eyelids were investigated in rat pups on the 14th postnatal day. They were processed histochemically for NADPH-d, to study the presence and distribution of nitric oxide synthase (NOS) positive nerve structures. NADPH-d staining of Meibomian glands was compared in two groups of rat pups. In the control group, eyelids of 14 day-old rats were studied with no experimental intervention. The second group consisted of rat pups which were prenatally administered the excess of RA. Histochemical analysis of control eyelids revealed numerous NADPH-d well-stained acini of Meibomian glands arranged tightly into groups. Intensively stained vessels and NADPH-d/NOS-positive nerve fibers bordered acini of Meibomian glands. These structures were present in the submucosal layer as well. The analysis of RA group showed less numerous, shrunken acini of Meibomian glands that were seen not only smaller in size, but also in density of their staining and the amount of nitrergic nerve fibers around acini were considerably lowered. In the submucosa differences were noticed compared to the control group, there were numerous NADPH-d stained vessels accompanied by NADPH-d/NOS-positive nerve fibers. The excess of RA during the prenatal period may influence on the development and morphology of NADPH-d positive structures of rat’s Meibomian glands.
机译：这项研究的目的是调查产前给予视黄酸（RA）对大鼠睑板腺烟酰胺腺嘌呤二核苷酸心肌黄递酶（NADPH-d）阳性结构发育的影响。在第12-14天的胚胎期，在妊娠期腹膜内将1 mg / kg的RA腹腔注射至怀孕的Wistar大鼠（总计3 mg / kg）。在出生后第14天，在大鼠幼崽中检查中央上眼睑的切片。对它们进行了NADPH-d的组织化学处理，以研究一氧化氮合酶（NOS）阳性神经结构的存在和分布。在两组大鼠幼崽中比较了睑板腺的NADPH-d染色。在对照组中，在没有实验干预的情况下研究了14日龄大鼠的眼睑。第二组由产前给予过量RA的幼鼠组成。对照眼睑的组织化学分析显示，许多NADPH-d染色良好的睑板腺腺瘤紧密排列。密集染色的血管和NADPH-d / NOS阳性神经纤维接壤于睑板腺的腺泡。这些结构也存在于粘膜下层。 RA组的分析显示，睑板腺的皱缩性腺泡数量不多，不仅尺寸更小，而且染色密度也大大降低，并且腺泡周围的硝化神经纤维数量明显减少。与对照组相比，在粘膜下层差异明显，有许多NADPH-d染色的血管伴有NADPH-d / NOS阳性神经纤维。产前时期过量的RA可能会影响大鼠睑板腺NADPH-d阳性结构的发育和形态。

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