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首页> 外文期刊>European Journal of Histochemistry >Effect of retinoic acid on the nitrergic innervation of meibomian glands in rats
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Effect of retinoic acid on the nitrergic innervation of meibomian glands in rats

机译：维甲酸对大鼠睑板腺硝化神经支配的影响

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摘要

The purpose of this study was to investigate the effect of prenatal administration of retinoic acid (RA) on the development of nicotinamide adenine dinucleotide diaphorase (NADPH-d) positive structures in the rat Meibomian glands. One mg/kg of RA was applied to pregnant Wistar rats intraperitonaelly during the gestational period in each of the 12~(th)-14~(th) embryonic days (totally 3 mg/kg). Sections of the central upper eyelids were investigated in rat pups on the 14~(th) postnatal day. They were processed histochemi-cally for NADPH-d, to study the presence and distribution of nitric oxide synthase (NOS) pos-itive nerve structures. NADPH-d staining of Meibomian glands was compared in two groups of rat pups. In the control group, eyelids of 14 day-old rats were studied with no experimental intervention. The second group consisted of rat pups which were prenatally administered the excess of RA. Histochemical analysis of control eyelids revealed numerous NADPH-d well-stained acini of Meibomian glands arranged tightly into groups. Intensively stained vessels and NADPH-d/NOS-positive nerve fibers bor-dered acini of Meibomian glands. These struc-tures were present in the submucosal layer as well. The analysis of RA group showed less numerous, shrunken acini of Meibomian glands that were seen not only smaller in size, but also in density of their staining and the amount of nitrergic nerve fibers around acini were considerably lowered. In the submucosa differences were noticed compared to the con-trol group, there were numerous NADPH-d stained vessels accompanied by NADPH-d/NOS-positive nerve fibers. The excess of RA during the prenatal period may influence on the devel-opment and morphology of NADPH-d positive structures of rat's Meibomian glands.
机译：这项研究的目的是调查产前给药视黄酸（RA）对大鼠睑板腺烟酰胺腺嘌呤二核苷酸心肌黄递酶（NADPH-d）阳性结构发育的影响。在孕期的第12（th）-14（th）天中，每天在妊娠期腹腔内向怀孕的Wistar大鼠施用1 mg / kg的RA（总计3 mg / kg）。在出生后第14天，对幼崽的中央上眼睑进行调查。对它们进行了NADPH-d的组织化学处理，以研究一氧化氮合酶（NOS）阳性神经结构的存在和分布。在两组大鼠幼崽中比较了睑板腺的NADPH-d染色。在对照组中，在没有实验干预的情况下研究了14日龄大鼠的眼睑。第二组由幼鼠组成，它们在产前被给予过量的RA。对照眼睑的组织化学分析显示，许多NADPH-d染色良好的睑板腺腺瘤紧密排列。强烈染色的血管和NADPH-d / NOS阳性神经纤维浸没了睑板腺的腺泡。这些结构也存在于粘膜下层。 RA组的分析显示，睑板腺的皱缩性腺泡数量不多，不仅尺寸更小，而且染色密度也大大降低，并且腺泡周围的硝化神经纤维数量明显减少。与对照组相比，在粘膜下层注意到差异，有许多NADPH-d染色的血管伴有NADPH-d / NOS阳性神经纤维。产前RA过量可能影响大鼠睑板腺NADPH-d阳性结构的发育和形态。

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