Immunogold study on lectin binding in the porcine zona pellucida and granulosa cells

F Parillo; C Dall’Aglio; A Verini Supplizi; P Ceccarelli; AM Gargiulo

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Immunogold study on lectin binding in the porcine zona pellucida and granulosa cells

机译：猪透明带和颗粒细胞中凝集素结合的免疫金研究

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An ultrastructural localization of lectin receptors on the zona pellucida (ZP) of porcine antral oocytes and on the granulosa cells was performed using a panel of horseradish peroxidase- labelled lectins in conjunction with antiperoxidase antibody and protein A-gold. In some cases, lectin incubation was preceded by sialidase digestion. WGA-, Con-A-, UEA-I-, RCA-I-, PNA- and SBA-reactive sites were distributed differently in the porcine ZP. Sialidase digestion increased the positivity obtained with RCA-I and it was necessary to promote PNA and SBA reactivity. These results indicated that the ZP contained N-acetylglucosamine, a-mannose, a- fucose, b-Gal-(1-4)GlcNAc, b-Gal- (1-3)GalNAc, b-GalNAc and sialic acid residues. We also observed the presence of vesicles in both the ooplasm and granulosa cells, showing a similar lectin binding pattern to that of the ZP, thus suggesting that the oocyte and granulosa cells are the site of synthesis of ZP glucidic determinants.

机译：使用一组辣根过氧化物酶标记的凝集素结合抗过氧化物酶抗体和蛋白A-金来进行凝集素受体在猪窦卵母细胞的透明带（ZP）和颗粒细胞上的超微结构定位。在某些情况下，凝集素孵育之前需要进行唾液酸酶消化。 WGA-，Con-A-，UEA-I，RCA-1-，PNA-和SBA反应位点在猪ZP中的分布不同。唾液酸酶消化增加了RCA-1获得的阳性，因此有必要促进PNA和SBA反应性。这些结果表明ZP含有N-乙酰基葡糖胺，α-甘露糖，α-岩藻糖，b-Gal-（1-4）GlcNAc，b-Gal-（1-3）GalNAc，b-GalNAc和唾液酸残基。我们还观察到卵泡和卵泡细胞中都存在囊泡，显示出与ZP相似的凝集素结合模式，因此表明卵母细胞和颗粒细胞是ZP糖决定簇的合成位点。

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《European Journal of Histochemistry》 |2003年第4期|共6页
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F Parillo; C Dall’Aglio; A Verini Supplizi; P Ceccarelli; AM Gargiulo;
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1. Immunogold study on lectin binding in the porcine zona pellucida and granulosa cells [J] . Parillo F., DallAglio C., Supplizi AV., European Journal of Histochemistry . 2003,第4期

机译：猪透明带和颗粒细胞中凝集素结合的免疫金研究
2. Inhibition of goat sperm-zona binding by monoclonal antibodies to a glycoprotein family (ZP4) of porcine zona pellucida. [J] . Gautam Kaul, Gandhi KK, Kaul G Small Ruminant Research . 2000,第2期

机译：单克隆抗体对猪透明带糖蛋白家族（ZP4）的单克隆抗体抑制山羊精子-zona结合。
3. Expression of a P-selectin Ligand in Zona Pellucida of Porcine Oocytes and P-selectin on Acrosomal Membrane of Porcine Sperm Cells. Potential Implications for Their Involvement in Sperm–Egg Interactions [J] . Jian-Guo Geng, Thomas J. Raub, Carolyn A. Baker, Journal of cell biology . 1997,第3期

机译：P-选择素配体在猪卵母细胞透明带中的表达以及P-选择素在猪精细胞顶体膜上的表达。它们参与精子-蛋相互作用的潜在意义
4. A Deeper Look Into Immature Porcine Zona Pellucida Visco-hyperelasticity [C] . A. Boccaccio, L. Lamberti, M. Papi, SEM International Congress Exposition on Experimental and Applied Mechanics . 2016

机译：深入了解未成熟的猪Zona Pellucida粘性粘度性超弹性
5. A comparison of the contraceptive potential of different immunologic presentations of a conjugated preparation of porcine zona pellucida in cow tule elk and domestic ewes. [D] . Stoops, Monica Ann. 2001

机译：猪透明带结合蛋白制剂在牛tu麋和家母羊中的不同免疫学表现的避孕潜力比较。
6. Expression of a P-selectin Ligand in Zona Pellucida of Porcine Oocytes and P-selectin on Acrosomal Membrane of Porcine Sperm Cells. Potential Implications for Their Involvement in Sperm–Egg Interactions [O] . Jian-Guo Geng, Thomas J. Raub, Carolyn A. Baker, 1997

机译：P-选择素配体在猪卵母细胞透明带中的表达以及P-选择素在猪精细胞顶体膜上的表达。它们参与精子-蛋相互作用的潜在意义
7. Immunogold study on lectin binding in the porcine zona pellucida and granulosa cells [O] . F Parillo, C Dall’Aglio, A Verini Supplizi, 2009

机译：猪透明带和颗粒细胞中凝集素结合的免疫金标记研究

Immunogold study on lectin binding in the porcine zona pellucida and granulosa cells

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