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首页> 外文期刊>European Journal of Histochemistry >The autonomous innervation of the buffalo (Bubalus bubalis) testis. An immunohistochemical study
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The autonomous innervation of the buffalo (Bubalus bubalis) testis. An immunohistochemical study

机译：水牛（Bubalus bubalis）睾丸的自主神经支配。免疫组织化学研究

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The innervation pattern in the buffalo testis was determined by using histochemical and immunohistochemical methods. Nerves were concentrated in the tunica albuginea and septula testis, and did not show an uniform distribution. The tunica albuginea at the lateral and medial sides and at the free border of the testis is most densely innervated than at the epididymal border. At the cranial pole thick nerve bundles were observed between albugineal vessels and muscle bundles. Rare parenchymal nerves were found in perivascular position between seminiferous tubules and their occurrence is confined to lobules at the cranial and caudal testicular poles. An intense NPY immunoreactivity occurred in nerve bundles and in solitary varicose fibres. Nerves were concentrated in the tunica albuginea at the lateral and medial side and at the free border of the testis, and in the lobules at the cranial and caudal testicular poles. Sub P immunoreactivity was occasionally detected in some thicker nerve bundles and solitary fibers, in the tunica albuginea and in the wall of blood vessels, showing a similar distribution but less intensity and density than NPY immunoreactivity. TH immunoreactivity stained nerve fibers in the buffalo testis with a distribution pattern similar to that obtained with general neuronal markers. The histochemical reaction for AchE was negative, so cholinergic fibers cannot be detected in the buffalo testis. The histochemical NADPHd reaction stained rare nitrergic nerve bundles and solitary fibers. The majority of NADPHd activity was confined to the vascular endothelium, and rarely to the interstitial Leydig cells, whereas the Sertoli and germ cells did not show any reaction.
机译：使用组织化学和免疫组织化学方法确定水牛睾丸中的神经支配模式。神经集中在白膜和睾丸中膜，未显示均匀分布。与睾丸边界相比，外侧外侧，内侧内侧以及睾丸自由边界的白膜最密集。在颅极处，在白血管和肌肉束之间观察到了粗大的神经束。在生精小管之间的血管周围位置发现稀少的实质神经，它们的发生局限于颅和尾睾丸极的小叶。在神经束和孤立的曲张纤维中发生强烈的NPY免疫反应。神经集中在白膜的外侧和内侧以及睾丸的自由边界，以及在颅和尾睾丸极的小叶中。在一些较厚的神经束和孤立纤维，白膜和血管壁中偶尔检测到Sub P免疫反应性，显示出与NPY免疫反应性相似的分布，但强度和密度较小。 TH免疫反应染色了水牛睾丸中的神经纤维，其分布模式与使用一般神经元标记获得的分布模式相似。 AchE的组织化学反应为阴性，因此在水牛睾丸中无法检测到胆碱能纤维。组织化学NADPHd反应染色罕见的硝化神经束和孤纤维。 NADPHd的大部分活性都局限于血管内皮，很少有间质Leydig细胞，而支持细胞和生殖细胞则没有任何反应。

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