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首页> 外文期刊>Andrologia >Immunohistochemical distribution of S-100 protein and subunits (S100-alpha and S100-beta) in the swamp-type water buffalo (Bubalus bubalis) testis.
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Immunohistochemical distribution of S-100 protein and subunits (S100-alpha and S100-beta) in the swamp-type water buffalo (Bubalus bubalis) testis.

机译：沼泽型水牛（Bubalus bubalis）睾丸中S-100蛋白和亚基（S100-alpha和S100-beta）的免疫组织化学分布。

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摘要

The distribution and localization of S-100 protein (S-100) and its subunits (S100-alpha and S100-beta) in the testis of swamp-type water buffalo were investigated using immunohistochemistry. S-100 was detected in the Sertoli cells in the convoluted seminiferous tubules, modified Sertoli cells lining the terminal segment of the seminiferous tubules and in the intratesticular excurrent ducts (straight tubules and rete testis). S100-beta showed the same distribution and localization with that of S-100. However, the cytoplasmic extension of the Sertoli cells in S100-beta staining showed less staining intensity compared with that of S-100. S100-alpha showed a positive staining only in the modified Sertoli cells of the terminal segment of the seminiferous tubule. Endothelial cells of blood vessels were also positive with the proteins while the Leydig and spermatogenic cells showed a negative reaction. The localization of S-100 in the testis of the water buffalo was in parallel with that of other artiodactylswhich supports the hypothesis that this protein is a multifunctional protein. S100-beta in the Sertoli cells suggests that this protein is involved in establishing blood-testis barrier. Its presence in the modified Sertoli cells and in the epithelium of the excurrent ducts suggest secretory and absorptive function, respectively. Meanwhile, S100-alpha, which was detected only in the modified Sertoli cells, is involved in the secretory activity of these cells that are related to exocrine function.
机译：利用免疫组织化学方法研究了沼泽水牛的睾丸中S-100蛋白（S-100）及其亚基（S100-alpha和S100-beta）的分布和定位。在弯曲的曲细精管中的支持细胞中检测到S-100，在曲细精管末端部分和睾丸内流出道（直小管和睾丸网）中发现了改良的支持细胞。 S100-beta具有与S-100相同的分布和定位。然而，与S-100相比，S100-beta染色中支持细胞的胞质延伸显示出较小的染色强度。 S100-alpha仅在生精小管末端部分的修饰Sertoli细胞中显示阳性染色。蛋白质的血管内皮细胞也呈阳性，而睾丸间质细胞和生精细胞则呈阴性反应。 S-100在水牛睾丸中的定位与其他动手动物的定位平行，这支持了该蛋白是一种多功能蛋白的假设。 Sertoli细胞中的S100-beta提示该蛋白与建立血液-睾丸屏障有关。它存在于修饰的支持细胞和外在导​​管的上皮中，分别提示其分泌和吸收功能。同时，仅在修饰的Sertoli细胞中检测到的S100-α参与了这些与外分泌功能有关的细胞的分泌活性。

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