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首页> 外文期刊>Experimental Animals >Comparative Proteomic Analyses of Macular and Peripheral Retina of Cynomolgus Monkeys (Macaca fascicularis)
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Comparative Proteomic Analyses of Macular and Peripheral Retina of Cynomolgus Monkeys (Macaca fascicularis)

机译：食蟹猴（Macaca fascicularis）的黄斑和周围视网膜的比较蛋白质组学分析。

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The central region of the primate retina is called the macula. The fovea is located at the center of the macula, where the photoreceptors are concentrated to create a neural network adapted for high visual acuity. Damage to the fovea, e.g., by macular dystrophies and age-related macular degeneration, can reduce central visual acuity. The molecular mechanisms leading to these diseases are most likely dependent on the proteins in the macula which differ from those in the peripheral retina in expression level. To investigate whether the distribution of proteins in the macula is different from the peripheral retina, proteomic analyses of tissues from these two regions of cynomolgus monkeys were compared. Two-dimensional gel electrophoresis and mass spectrometry identified 26 proteins that were present only in the macular gel spots. The expression levels of five proteins, cone photoreceptor specific arrestin-C, γ-synuclein, epidermal fatty acid binding protein, tropomyosin 1α chain, and heterogeneous nuclear ribonucleoproteins A2/B1, were significantly higher in the macula than in the peripheral retina. Immunostaining of macula sections by antibodies to each identified protein revealed unique localization in the retina, retinal pigment epithelial cells and the choroidal layer. Some of these proteins were located in cells with higher densities in the macula. We suggest that it will be important to study these proteins to determine their contribution to the pathogenesis and progression of macula diseases.
机译：灵长类动物视网膜的中央区域称为黄斑。中央凹位于黄斑中心，光感受器在那里集中，形成一个适合于高视敏度的神经网络。例如，由黄斑营养不良和与年龄有关的黄斑变性所引起的对中央凹的损害可降低中心视力。导致这些疾病的分子机制很可能取决于黄斑中表达水平与周围视网膜蛋白不同的蛋白。为了研究黄斑中蛋白质的分布与周围视网膜是否不同，比较了食蟹猴这两个区域组织的蛋白质组学分析。二维凝胶电泳和质谱鉴定了仅存在于黄斑凝胶斑点中的26种蛋白质。与黄斑区相比，黄斑区的5种蛋白，视锥细胞特异性视紫红质抑制蛋白-C，γ-突触核蛋白，表皮脂肪酸结合蛋白，原肌球蛋白1α链和异质核核糖核蛋白A2 / B1的表达水平明显更高。通过针对每种鉴定出的蛋白质的抗体对黄斑切片进行免疫染色，揭示了视网膜，视网膜色素上皮细胞和脉络膜层的独特定位。这些蛋白质中的一些位于黄斑中密度较高的细胞中。我们建议研究这些蛋白质以确定它们对黄斑疾病的发病机理和进展的贡献将是重要的。

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