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首页> 外文期刊>Experimental Animals >Subchondral bone derived mesenchymal stem cells display enhanced osteo-chondrogenic differentiation, self-renewal and proliferation potentials
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Subchondral bone derived mesenchymal stem cells display enhanced osteo-chondrogenic differentiation, self-renewal and proliferation potentials

机译：软骨下骨来源的间充质干细胞显示出增强的骨软骨分化，自我更新和增殖潜能

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Rabbit mesenchymal stem cells (MSCs) are important seed cells in regenerative medicine research, particularly in translational research. In the current study, we showed that rabbit subchondral bone is a reliable source of MSCs. First, we harvested subchondral bone (SCB) from the rabbit knee-joint and initiated the MSC culture by cultivating enzyme-treated SCB. Adherent fibroblast-like cells that outgrew from SCB fulfill the common immuno-phenotypic criteria for defining MSCs, but with low contamination of CD45+ hematopoietic cells. Interestingly, differentiated SCB-MSCs expressed osteogenic and chondrogenic markers at significantly higher levels than those in bone marrow cell suspension-derived MSCs (BMS-MSCs) (P0.05). No differences in the expression of adipogenic markers between SCB-MSC and BMS-MSC (P0.05) were observed. Moreover, the results of the colony forming unit-fibroblast assay and sphere formation assay demonstrated that the SCB-MSCs had increased self-renewal potential. SCB-MSCs expressed higher levels of the stemness markers Nanog, OCT4, and Sox-2 compared to in BMS-MSCs (P0.05). Furthermore, the results of both the CCK-8-based assay and CFSE dilution assay showed that SCB-MSCs exhibited enhanced proliferative capacity. In addition, SCB-MSCs exhibited higher phosphorylation of extracellular signal-related kinase/mitogen-activated protein kinase signaling, which is closely related to MSC proliferation. In conclusion, we identified SCB-MSCs as a novel stem cell population that met the requirements of MSCs; the unique properties of SCB-MSC are important for the potential treatment of tissue damage resulting from disease and trauma.
机译：兔间充质干细胞（MSC）是再生医学研究（尤其是转化研究）中重要的种子细胞。在当前的研究中，我们表明兔软骨下骨是MSC的可靠来源。首先，我们从兔膝关节中收获软骨下骨（SCB），并通过培养酶处理的SCB来启动MSC培养。从SCB中长出的贴壁成纤维样细胞满足定义MSC的常见免疫表型标准，但CD45 +造血细胞的污染低。有趣的是，分化的SCB-MSCs的成骨和软骨生成标记物的表达水平明显高于骨髓细胞悬液来源的MSCs（BMS-MSCs）（P <0.05）。 SCB-MSC和BMS-MSC之间脂肪形成标记的表达没有差异（P> 0.05）。此外，菌落形成单位-成纤维细胞测定和球形成测定的结果表明，SCB-MSC具有增加的自我更新潜能。与BMS-MSCs相比，SCB-MSCs表达的干度标记物Nanog，OCT4和Sox-2水平更高（P <0.05）。此外，基于CCK-8的测定和CFSE稀释测定的结果均显示SCB-MSC表现出增强的增殖能力。此外，SCB-MSCs表现出较高的细胞外信号相关激酶/促分裂原活化蛋白激酶信号转导的磷酸化，这与MSC增殖密切相关。总之，我们将SCB-MSCs鉴定为满足MSCs要求的新型干细胞群体。 SCB-MSC的独特特性对于潜在治疗因疾病和创伤引起的组织损伤非常重要。

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