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首页> 外文期刊>Eukaryotic cell >Evidence of mRNA-Mediated Intron Loss in the Human-Pathogenic Fungus Cryptococcus neoformans
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Evidence of mRNA-Mediated Intron Loss in the Human-Pathogenic Fungus Cryptococcus neoformans

机译：在人类致病性真菌新隐球菌中mRNA介导的内含子丢失的证据。

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Introns are a defining feature of eukaryotic genomes, though the mechanism of intron gain or loss is not well understood. Reverse transcription of mRNA followed by homologous recombination with the genome has been posited as a mechanism of intron loss, though little direct evidence of recent loss events has been described to support this model. We find supporting evidence for an mRNA-mediated mechanism of loss through comparative genome analyses that revealed a recent loss of 10 adjacent introns in a 22-exon gene in the human-pathogenic fungus Cryptococcus neoformans. We surveyed the gene structures of the entire genomes of Cryptococcus gattii, which diverged from the C. neoformans lineage 37 million years ago (Mya), and C. neoformans var. grubii and var. neoformans, which diverged 18 Mya. Our comparison revealed greater than 99.9% intron conservation, with evidence from 20 genes showing evidence of intron loss, but no convincing evidence of intron gain. Our findings confirm that Cryptococcus introns have been quite stable over recent evolutionary time, with occasional mRNA-mediated intron loss events.
机译：内含子是真核生物基因组的定义特征，尽管对内含子获得或缺失的机理尚不十分了解。已经证实，mRNA的逆转录以及与基因组的同源重组是内含子丢失的机制，尽管最近很少有直接丢失事件的直接证据被描述来支持该模型。我们通过比较基因组分析发现了mRNA介导的丢失机制的支持证据，该分析揭示了人类致病性真菌“新隐球菌”中22个外显子基因中最近丢失了10个相邻内含子。我们调查了 Cryptococcus gattii 的整个基因组的基因结构，这些基因与 C有所不同。 3千3百万年前（Mya）的新形成族谱系和 C。新甲虫 var。 grubii 和var。 neoformans ，其中有18个Mya。我们的比较显示了超过99.9％的内含子保守性，来自20个基因的证据显示内含子丢失，但没有令人信服的内含子获得证据。我们的研究结果证实，隐球菌内含子在最近的进化时间内一直非常稳定，偶有mRNA介导的内含子丢失事件。

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