...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Eukaryotic cell >SWI/SNF Displaces SAGA-Acetylated Nucleosomes
【24h】

SWI/SNF Displaces SAGA-Acetylated Nucleosomes

机译：SWI / SNF取代SAGA乙酰化的核小体

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

SWI/SNF is a well-characterized chromatin remodeling complex that remodels chromatin by sliding nucleosomes in cis and/or displacing nucleosomes in trans. The latter mechanism has the potential to remove promoter nucleosomes, allowing access to transcription factors and RNA polymerase. In vivo, histone acetylation often precedes apparent nucleosome loss; therefore, we sought to determine whether nucleosomes containing acetylated histones could be displaced by the SWI/SNF chromatin remodeling complex. We found that SAGA-acetylated histones were lost from an immobilized nucleosome array when treated with the SWI/SNF complex. When the nucleosome array was acetylated by SAGA in the presence of bound transcription activators, it generated a peak of acetylation surrounding the activator binding sites. Subsequent SWI/SNF treatment suppressed this acetylation peak. Immunoblots indicated that SWI/SNF preferentially displaced acetylated histones from the array relative to total histones. Moreover, the Swi2/Snf2 bromodomain, an acetyl-lysine binding domain, played a role in the displacement of acetylated histones. These data indicate that targeted histone acetylation by the SAGA complex predisposes promoter nucleosomes for displacement by the SWI/SNF complex.
机译：SWI / SNF是一种很好表征的染色质重塑复合物，它通过在 cis 中滑动核小体和/或在 trans 中置换核小体来重塑染色质。后一种机制具有去除启动子核小体的潜力，从而允许使用转录因子和RNA聚合酶。在体内，组蛋白乙酰化通常先于明显的核小体丢失。因此，我们试图确定含有乙酰化组蛋白的核小体是否可以被SWI / SNF染色质重塑复合体所取代。我们发现，用SWI / SNF复合物处理时，SAGA乙酰化的组蛋白从固定的核小体阵列中丢失。当核糖体阵列在结合的转录激活剂存在下被SAGA乙酰化时，会在激活剂结合位点周围产生一个乙酰化峰。随后的SWI / SNF处理抑制了该乙酰化峰。免疫印迹表明，相对于总组蛋白，SWI / SNF优先从阵列中置换乙酰化组蛋白。此外，Swi2 / Snf2溴结构域，一个乙酰赖氨酸结合域，在乙酰化组蛋白的置换中发挥了作用。这些数据表明，SAGA复合物的靶向组蛋白乙酰化使启动子核小体易于被SWI / SNF复合物置换。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号