Is the MLC1/3 promoter / enhancer the right systemto generate skeletal muscle specific transgenicanimals?

Dr. Thomas Walther

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Is the MLC1/3 promoter / enhancer the right systemto generate skeletal muscle specific transgenicanimals?

机译：MLC1 / 3启动子/增强子是产生骨骼肌特异性转基因动物的正确系统吗？

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To document the role of iNOS expression for skeletal muscle alterations as observed in patients with chronic heart failure, the aim of this study was to generate skeletal muscle specific iNOS transgenic animals. The complete coding cDNA was inserted into a vector using the MLC-1/3 promoter/enhancer, which was used for pronuclear microinjection into 223 oocytes. PCR and Southern blot analysis of tail biopsies of 37 founder animals demonstrated that none was positive for the transgene. RT-PCR analysis of bovine oocytes for the expression of myosin light chain-1 revealed a positive amplification product. These results demonstrate for the first time that MLC-1 is already expressed in oocytes thus preventing the generation of transgenic animals using the MLC-1/3 promoter in conjunction with a toxic gene like iNOS.

机译：为了证明在慢性心力衰竭患者中观察到的iNOS表达对于骨骼肌改变的作用，本研究的目的是生成骨骼肌特异性iNOS转基因动物。使用MLC-1 / 3启动子/增强子将完整的编码cDNA插入载体，将其用于原核显微注射到223个卵母细胞中。对37只创始动物的尾巴活检进行PCR和Southern印迹分析表明，转基因没有阳性。牛卵母细胞表达肌球蛋白轻链1的RT-PCR分析显示阳性扩增产物。这些结果首次证明了MLC-1已经在卵母细胞中表达，因此使用MLC-1 / 3启动子结合有毒基因（如iNOS）阻止了转基因动物的产生。

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《Gene Therapy and Molecular Biology》 |2004年第2期|共6页
作者
Dr. Thomas Walther;
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中图分类基础医学;
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专利

1. DEVELOPMENTAL AND TISSUE-SPECIFIC REGULATION OF THE MURINE CARDIAC ACTIN GENE IN VIVO DEPENDS ON DISTINCT SKELETAL AND CARDIAC MUSCLE-SPECIFIC ENHANCER ELEMENTS IN ADDITION TO THE PROXIMAL PROMOTER [J] . Biben C., Tajbakhsh S., Buckingham M., Developmental biology . 1996,第1期

机译：体外，离体骨骼和心脏特定肌增强元素对小鼠心脏心肌基因的发育和组织特异性调节
2. Developmental and Tissue-Specific Regulation of the Murine Cardiac Actin Genein VivoDepends on Distinct Skeletal and Cardiac Muscle-Specific Enhancer Elements in Addition to the Proximal Promoter [J] . Christine Biben, Juliette Hadchouel, Shahragim Tajbakhsh, Developmental biology . 1996,第1期

机译：小鼠心脏肌动蛋白基因在体内的发育和组织特异性调节取决于除近端启动子外骨骼和心脏肌肉特异性增强子的组成
3. A skeletal muscle-specific enhancer regulated by factors binding to E and CArG boxes is present in the promoter of the mouse myosin light-chain 1A gene. [J] . F Catala, R Wanner, P Barton, Molecular and Cellular Biology . 1995,第8期

机译：小鼠肌球蛋白轻链1A基因的启动子中存在受与E和CArG框结合的因子调控的骨骼肌特异性增强子。
4. Changes in the expression of selected proteins elucidate skeletal muscle type-specific resistance to glucocorticoid-induced muscle cachexia [C] . P. Pawlikowska, M. Lokociejewska, J.F. Hocquette, International Symposium on Energy and Protein Metabolism and Nutrition . 2007

机译：所选蛋白质表达的变化阐明骨骼肌型特异性对糖皮质激素诱导的肌肉恶病症的抗性
5. Fast skeletal muscle fiber-type-specificity of the troponin I (fast) gene IRE enhancer resides in a 30 base-pair region [D] . Kumar, Angela 2003

机译：肌钙蛋白I（快速）基因IRE增强子的快速骨骼肌纤维类型特异性位于30个碱基对区域
6. A skeletal muscle-specific enhancer regulated by factors binding to E and CArG boxes is present in the promoter of the mouse myosin light-chain 1A gene. [O] . F Catala, R Wanner, P Barton, 1995

机译：小鼠肌球蛋白轻链1A基因的启动子中存在受与E和CArG框结合的因子调控的骨骼肌特异性增强子。
7. Developmental and Tissue-Specific Regulation of the Murine Cardiac Actin Genein VivoDepends on Distinct Skeletal and Cardiac Muscle-Specific Enhancer Elements in Addition to the Proximal Promoter [O] . Biben Christine, Hadchouel Juliette, Tajbakhsh Shahragim, 1996

机译：小鼠心脏肌动蛋白基因在体内的发育和组织特异性调节取决于除近端启动子外骨骼和心肌特异性的增强子元素

Is the MLC1/3 promoter / enhancer the right systemto generate skeletal muscle specific transgenicanimals?

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