...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Genes and Nutrition >Soy pinitol acts partly as an insulin sensitizer or insulin mediator in 3T3-L1 preadipocytes
【24h】

Soy pinitol acts partly as an insulin sensitizer or insulin mediator in 3T3-L1 preadipocytes

机译：大豆松醇在3T3-L1前脂肪细胞中部分充当胰岛素增敏剂或胰岛素介质

获取原文
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The blood glucose-lowering property of pinitol is mediated via the insulin signaling pathway. This study was carried out to evaluate the effects of soy pinitol on adipogenesis in a 3T3-L1 cell line; 3T3-L1 preadipocytes were treated with pinitol (0–1?mM) together with insulin for 9?days. The regulation of lipid metabolism was assessed by oil-red-O staining of intracellular lipids and real-time PCR of adipogenesis-related factors. The inhibition of cell proliferation was estimated by MTT assay. Pinitol treatment did not inhibit lipid accumulation, nor did it affect expression of adipogenesis-related factors, including ADD1, aP2 and FAS, in a dose-dependent manner. Expression of adiponectin, GLUT4, IRS, C/EBPα and PPARγ mRNAs, however, increased in cells treated with 0.5?mM and/or 1?mM pinitol. Pinitol treatment did not affect the inhibition of cell growth and proliferation in a dose-dependent manner. Accordingly, we suggest that pinitol is nontoxic to this cell line, and that it enhances adipogenesis by acting as an insulin sensitizer or insulin mediator via the upregulation of adiponectin, GLUT4, IRS, C/EBPα and PPARγ in 3T3-L1 preadipocytes.
机译：松醇的降血糖特性是通过胰岛素信号传导途径介导的。进行这项研究以评估大豆松醇对3T3-L1细胞系中脂肪形成的影响。 3T3-L1前脂肪细胞用松醇（0–1？mM）和胰岛素一起治疗9天。脂质代谢的调节通过细胞内脂质的油红O染色和脂肪形成相关因子的实时PCR进行评估。通过MTT测定估计细胞增殖的抑制。松醇治疗并没有抑制脂质蓄积，也没有以剂量依赖的方式影响脂肪形成相关因子（包括ADD1，aP2和FAS）的表达。然而，脂联素，GLUT4，IRS，C /EBPα和PPARγmRNA的表达在用0.5？mM和/或1？mM松果糖醇处理的细胞中增加了。松醇治疗不以剂量依赖的方式影响细胞生长和增殖的抑制。因此，我们认为松醇对这种细胞系无毒，并且它通过在3T3-L1前脂肪细胞中通过脂联素，GLUT4，IRS，C /EBPα和PPARγ的上调而充当胰岛素敏化剂或胰岛素介体，从而增强脂肪生成。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号