...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Genetics and Molecular Research >Isolation and characterization of simple sequence repeat markers for the herbaceous species Phyla scaberrima (Verbenaceae)
【24h】

Isolation and characterization of simple sequence repeat markers for the herbaceous species Phyla scaberrima (Verbenaceae)

机译：草本植物Phyla scaberrima（Verbenaceae）的简单序列重复标记的分离与鉴定

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Phyla scaberrima (Verbenaceae) is a herbaceous species distributed from Mexico to Panama. Because of its well-known sweet properties and other medicinal uses, this species is cultivated in South America and the Caribbean. Phyla scaberrima has been arbitrarily extracted from nature, resulting in a severe reduction in its gene pool. In this study, we developed and characterized 11 simple sequence repeat markers for P. scaberrima to determine the genetic variability and patterns of population structure of the species. Fifty-six alleles were detected in a sample of 48 individuals belonging to 3 different populations. The average number of alleles per locus was 5.09, while the polymorphic information content ranged from 0.000-0.587. The observed and expected heterozygosities varied from 0.000-0.543 and from 0.000-0.651, respectively. Two loci exhibited significant deviation of the expected Hardy-Weinberg proportion. The 11 primer pairs were also tested for cross-amplification to 6 species of the related genus Lippia. The transferability rate ranged from 4 loci in Lippia florida and L. rotundifolia to 6 loci in L. corymbosa and L. microcephala. The 11 primer sets were shown to be valuable tools for population genetic studies in P. scaberrima and in species of the genus Lippia in which primer transferability was detected.
机译：Phyla scaberrima（马鞭草科）是一种从墨西哥分布到巴拿马的草本植物。由于其众所周知的甜味特性和其他医学用途，该物种在南美和加勒比海种植。稻草（Phyla scaberrima）已从自然界中任意提取，导致其基因库大量减少。在这项研究中，我们开发和表征了11个简单的序列重复标记，用于确定鳞茎假单胞菌的遗传变异性和种群结构的模式。在属于3个不同人群的48个个体的样本中检测到56个等位基因。每个基因座的平均等位基因数目为5.09，而多态信息含量范围为0.000-0.587。观察到的和期望的杂合度分别为0.000-0.543和0.000-0.651。两个基因座表现出预期的Hardy-Weinberg比例的显着偏差。还测试了这11对引物对相关6个物种Lippia属的交叉扩增。迁移率的范围从佛罗里达的Lippia和圆叶的L. rotundifolia的4个基因座到Co.mbosa和L. microcephala的6个基因座。结果表明，这11个引物组是进行斑节对虾和Lippia属物种中种群遗传学研究的有价值的工具，在其中检测到了引物可转移性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号