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首页> 外文期刊>Biomass & bioenergy >Isolation and characterization of simple sequence repeat loci in Miscanthus floridulus and their potential use as markers in related species
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Isolation and characterization of simple sequence repeat loci in Miscanthus floridulus and their potential use as markers in related species

机译：花芒草中简单序列重复基因座的分离与鉴定及其在相关物种中的标记潜力

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摘要

Twelve polymorphic simple sequence repeat (SSR) loci were isolated and confirmed in Miscanthus floridulus using the FIASCO (fast isolation of AFLP sequences containing repeats) protocol. Alleles of each locus were characterized in four populations of M. floridulus collected in the Liangzi lake area of Wuhan, China. The heterozygosity of the analyzed markers varied from 0.351 to 0.855, with the observed alleles at each locus ranging from 3 to 12 (with an average of 7.8). Cross-species polymorphisms were examined in five other species, including Miscanthus sinensis, Miscanthus sacchariflorus, Miscanthus lutarioriparius, Miscanthus x giganteus and Narenga porphyrocoma. Among the 12 tested SSR markers, all were successfully amplified in M. sinensis, 11 were amplified in M. sacchariflorus, M. lutarioriparius and M. x giganteus, and 7 were amplified in N. porphyrocoma.
机译：分离了十二个多态性简单序列重复（SSR）基因座，并使用FIASCO（包含重复序列的AFLP序列的快速分离）方案在芒草中进行了确认。每个位点的等位基因在中国武汉市梁子湖地区采集到的四个花支分枝杆菌种群中进行了表征。分析标记的杂合度在0.351到0.855之间变化，在每个位点观察到的等位基因在3到12之间（平均7.8）。跨物种多态性在五个其他物种中进行了检查，包括中华芒草，蔗糖芒草，lutlutorioriparius芒草，x giganteus芒草和纳氏藻。在12个测试的SSR标记中，所有标记均已在中华绒螯蟹中成功扩增，其中11个在糖摩氏螺菌，lutarioriparius和M. x giganteus中扩增，其中7个在紫藻中扩增。

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  • 来源
    《Biomass & bioenergy》 |2012年第11期|801-804|共4页
  • 作者

    Xiaohu Hu; Ying Diao; Xingfei Zheng; Zhen Qu; Fasong Zhou; Zhongli Hu;

  • 作者单位

    State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Wuhan, Hubei 430072, China;

    State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Wuhan, Hubei 430072, China;

    State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Wuhan, Hubei 430072, China;

    State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Wuhan, Hubei 430072, China;

    State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Wuhan, Hubei 430072, China;

    State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Wuhan, Hubei 430072, China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    energy crop; miscanthus; SSR; cross-species amplification; potential use;

    机译：能源作物can SSR;跨物种扩增;潜在用途;

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