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Effect of domestication on microorganism diversity and anaerobic digestion of food waste

机译：驯化对餐厨垃圾微生物多样性和厌氧消化的影响

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To accomplish the rapid start-up and stable operation of biogas digesters, an efficient inoculum is required. To obtain such an inoculum for food waste anaerobic digestion, we domesticated dairy manure anaerobic digestion residue by adding food waste every day. After 36 days, the pH and biogas yield stabilized signifying the completion of domestication. During domestication, the microbial communities in the inocula were investigated by constructing 16S rDNA clone libraries. We evaluated the effect of the domesticated inoculum by testing batch food waste anaerobic digestion with a non-domesticated inoculum as a control. The pH and methane yield of the digestion systems were determined as measurement indices. Domestication changed the composition and proportion of bacteria and archaea in the inocula. Of the bacteria, Clostridia (49.3%), Bacteroidales (19.5%), and Anaerolinaceae (8.1%) species were dominant in the seed sludge; Anaerolinaceae (49.0%), Clostridia (28.4%), and Bacteroidales (9.1%), in domestication sludge. Methanosaeta was the dominant genus in both of the seed (94.3%) and domestication (74.3%) sludge. However, the diversity of methanogenic archaea was higher in the domestication than in seed sludge. Methanoculleus, which was absent from the seed sludge, appeared in the domestication sludge (21.7%). When the domesticated inoculum was used, the digestion system worked stably (organic loading rate: 20 gVS/L; methane yield: 292.2 ± 9.8 mL/gVS; VS = volatile solids), whereas the digestion system inoculated with seed sludge failed to generate biogas. The results indicate that inoculum domestication ensures efficient and stable anaerobic digestion by enriching the methanogenic strains.
机译：为了实现沼气池的快速启动和稳定运行，需要高效的接种物。为了获得用于食物垃圾厌氧消化的接种物，我们通过每天添加食物垃圾来驯化奶牛粪的厌氧消化残留物。 36天后，pH和沼气产量稳定，这表明驯化已完成。在驯化过程中，通过构建16S rDNA克隆文库研究了接种物中的微生物群落。我们通过以非驯化接种物为对照测试分批食物垃圾厌氧消化来评估驯化接种物的效果。测定消化系统的pH和甲烷产率作为测量指标。驯化改变了接种物中细菌和古细菌的组成和比例。在细菌中，梭状芽胞杆菌（49.3％），拟杆菌（19.5％）和Anaerolinaceae（8.1％）在种子污泥中占优势。驯化污泥中的厌氧菌科（49.0％），梭菌（28.4％）和细菌杆菌（9.1％）。在种子污泥（94.3％）和驯化污泥（74.3％）中，甲烷菌是最主要的属。但是，驯化中产甲烷古菌的多样性高于种子污泥。种子污泥中不存在的甲烷菌出现在驯化污泥中（21.7％）。当使用驯化接种物时，消化系统运行稳定（有机负载率：20 gVS / L;甲烷产量：292.2±9.8 mL / gVS; VS =挥发性固体），而接种有种子污泥的消化系统无法产生沼气。结果表明，接种物驯化可通过富集产甲烷菌株确保有效和稳定的厌氧消化。

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