...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Genetics and Molecular Research >Enhancement of dendritic cells with melanoma-associated antigen 3 for inducing cytotoxicity by cytotoxic T lymphocytes on bladder cancer BIU-87 cells
【24h】

Enhancement of dendritic cells with melanoma-associated antigen 3 for inducing cytotoxicity by cytotoxic T lymphocytes on bladder cancer BIU-87 cells

机译：黑色素瘤相关抗原3增强树突状细胞，以通过细胞毒性T淋巴细胞诱导膀胱癌BIU-87细胞发生细胞毒性

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

To determine the cytotoxic effect of lymphocytes activated by melanoma-associated antigen 3 (MAGE-3)-sensitized dendritic cells (DCs) on BIU-87 tumor cells, and to evaluate the possibility of MAGE-3-peptide-pulsed DCs as a vaccine in bladder cancer immunotherapy, the proliferation of T cells and the activity of cytotoxic T lymphocytes (CTLs) were examined by the MTT method. CTLs were induced by MAGE-3-sensitized DCs, or by ovalbumin (OVA) peptide and non-sensitized DCs as controls, respectively. The results indicated that MAGE-3-sensitized DCs have the ability to promote the proliferation of T cells as well as the cytotoxic activity of CTLs on bladder cancer cells in comparison with OVA peptide and non-sensitized DCs. In other words, DCs sensitized by the MAGE-3 antigen peptide could obviously upregulate the proliferation of T cells, which resulted in the growth inhibition of bladder cancer BIU-87 cells. In addition, MAGE-3-sensitized DCs played an important role in inhibiting the growth of human BIU-87 tumor xenografts in nude mice.
机译：确定由黑色素瘤相关抗原3（MAGE-3）致敏的树突状细胞（DC）激活的淋巴细胞对BIU-87肿瘤细胞的细胞毒作用，并评估MAGE-3肽脉冲DC作为疫苗的可能性在膀胱癌免疫疗法中，MTT法检测了T细胞的增殖和细胞毒性T淋巴细胞（CTL）的活性。 CTL由MAGE-3致敏的DC诱导，或由卵清蛋白（OVA）肽和非致敏的DC诱导作为对照。结果表明，与OVA肽和未敏化的DC相比，MAGE-3敏化的DC具有促进T细胞增殖以及CTL对膀胱癌细胞的细胞毒活性的能力。换句话说，MAGE-3抗原肽致敏的DCs明显上调T细胞的增殖，从而导致膀胱癌BIU-87细胞的生长受到抑制。此外，MAGE-3敏感的DC在抑制裸鼠中人BIU-87肿瘤异种移植的生长中起着重要作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号