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Improved Raoultella planticola Strains for the Production of 2,3-Butanediol from Glycerol

机译:从甘油生产2,3-丁二醇的改良Raoultella planticola菌株

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Raw glycerol is an industrial byproduct from biodiesel production and is one of the most promising substrates for 2,3-butanediol (2,3-BD) production; however, 2,3-BD is not yet produced by fermentation from glycerol on a commercial scale due to poor process economics. Class 1 microorganism collections were screened and Raoultella planticola strain CECT 843 proved to be the best 2,3-BD producer, achieving (23.3 ± 1.4) g 2,3-BD per L and a yield of 36% (g 2,3-BD per g glycerol). To further increase product concentration and yield, R. planticola CEC T843 was subjected to random mutagenesis using ultra-violet (UV) light and ethyl methane sulfonate (EMS). Two mutant strains were found to produce at least 30% more 2,3-BD than the wild type: R. planticola IA1 [(30.8 ± 3.9) g 2,3-BD per L and 49% yield] and R. planticola IIIA3 [(30.5 ± 0.4) g 2,3-BD per L and 49% yield].
机译:粗甘油是生物柴油生产的工业副产品,是2,3-丁二醇(2,3-BD)生产中最有前途的底物之一。然而,由于工艺经济性差,还没有以商业规模由甘油发酵生产2,3-BD。筛选了1类微生物收集物,劳氏菌CECT 843菌株被证明是最好的2,3-BD生产者,每升达到(23.3±1.4)g 2,3-BD,收率为36%(g 2,3-g) BD / g甘油)。为了进一步提高产品浓度和产量,使用紫外线(UV)和甲烷磺酸乙酯(EMS)对植物R. CEC T843进行了随机诱变。发现两个突变株比野生株产生的2,3-BD至少多出30%:R。planticola IA1 [(30.8±3.9)g 2,3-BD / L和49%的产率]和R. planticola IIIA3 [(30.5±0.4)g 2,3-BD / L,产率为49%]。

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