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首页> 外文期刊>Food Science & Nutrition >Probiotic potentials of Lactobacillus plantarum isolated from fermented durian (Tempoyak), a Malaysian traditional condiment
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Probiotic potentials of Lactobacillus plantarum isolated from fermented durian (Tempoyak), a Malaysian traditional condiment

机译：从马来西亚传统调味品榴莲（Tempoyak）中分离出来的植物乳杆菌的益生菌潜力

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Lactic acid bacterium isolated from fermented durian (tempoyak) was investigated for its potentials as a probiotic strain. Bacterial tolerance toward gastrointestinal environment, adhesion, and cytotoxic activity in human colon adenocarcinoma cell line HT‐29 was evaluated. 16S rRNA sequencing identified the lactic acid bacterium as Lactobacillus plantarum . The bacterium demonstrated good tolerance toward gastrointestinal pH 2.0 and 0.3% bile salts. It showed strong adhesive capacity in human intestinal cell line, HT‐29, with an adhesion index of 159?±?10. Cytotoxicity of L.?plantarum was investigated using both live bacterial cells (BC) and cell‐free supernatant (CFS). Findings showed that both BC and CFS of L.?plantarum reduced proliferation of HT‐29 colon adenocarcinoma cells using MTT assay. The results imply potential probiotic properties of L.?plantarum isolated from tempoyak.
机译：从发酵的榴莲（tempoyak）中分离出的乳酸菌具有作为益生菌菌株的潜力。评估了人类结肠腺癌细胞系HT‐29对胃肠道环境的细菌耐受性，粘附性和细胞毒性活性。 16S rRNA测序确定乳酸菌为植物乳杆菌。该细菌表现出对胃肠道pH 2.0和0.3％胆汁盐的良好耐受性。它在人肠道细胞HT‐29中显示出强大的粘附能力，粘附指数为159？±？10。使用活细菌细胞（BC）和无细胞上清液（CFS）研究了植物乳杆菌的细胞毒性。研究结果表明，使用MTT法测定植物乳杆菌的BC和CFS均可降低HT-29结肠腺癌细胞的增殖。该结果暗示从tempoyak分离的植物乳杆菌的潜在益生菌性质。

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