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首页> 外文期刊>Food Science & Nutrition >Antibiotic resistance and multidrug-resistant efflux pumps expression in lactic acid bacteria isolated from pozol, a nonalcoholic Mayan maize fermented beverage
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Antibiotic resistance and multidrug-resistant efflux pumps expression in lactic acid bacteria isolated from pozol, a nonalcoholic Mayan maize fermented beverage

机译：从非酒精玛雅玉米发酵饮料pozol分离出的乳酸菌中，抗生素抗性和多药外排泵的表达

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Abstract Pozol is a handcrafted nonalcoholic Mayan beverage produced by the spontaneous fermentation of maize dough by lactic acid bacteria. Lactic acid bacteria (LAB) are carriers of chromosomal encoded multidrug-resistant efflux pumps genes that can be transferred to pathogens and/or confer resistance to compounds released during the fermentation process causing food spoiling. The aim of this study was to evaluate the antibiotic sensibility and the transcriptional expression of ABC-type efflux pumps in LAB isolated from pozol that contributes to multidrug resistance. Analysis of LAB and Staphylococcus ( S .) aureus ATCC 29213 and ATCC 6538 control strains to antibiotic susceptibility, minimal inhibitory concentration (MIC), and minimal bactericidal concentration (MBC) to ethidium bromide were based in ?￠????standard methods?￠???? whereas the ethidium bromide efflux assay was done by fluorometric assay. Transcriptional expression of efflux pumps was analyzed by RT-PCR. LAB showed antibiotic multiresistance profiles, moreover, Lactococcus ( L .) lactis and Lactobacillus ( L .) plantarum displayed higher ethidium bromide efflux phenotype than S . aureus control strains. Ethidium bromide resistance and ethidium bromide efflux phenotypes were unrelated with the overexpression of lmrD in L . lactics , or the underexpression of lmrA in L . plantarum and norA in S . aureus . These findings suggest that, moreover, the analyzed efflux pumps genes, other unknown redundant mechanisms may underlie the antibiotic resistance and the ethidium bromide efflux phenotype in L . lactis and L . plantarum . Phenotypic and molecular drug multiresistance assessment in LAB may improve a better selection of the fermentation starter cultures used in pozol, and to control the antibiotic resistance widespread and food spoiling for health safety.
机译：摘要Pozol是一种手工制作的非酒精玛雅饮料，它是由乳酸菌自发发酵玉米面团而制得的。乳酸菌（LAB）是染色体编码的多药耐药性外排泵基因的载体，这些基因可以转移到病原体和/或赋予在发酵过程中释放的导致食物变质的化合物的抗性。这项研究的目的是评估从波佐尔分离的LAB中的抗生素敏感性和ABC型外排泵的转录表达，这有助于多药耐药性。 LAB和金黄色葡萄球菌ATCC 29213和ATCC 6538对照菌株对抗生素敏感性，对溴化乙锭的最小抑菌浓度（MIC）和最小杀菌浓度（MBC）的分析均采用标准方法。 ￠ ????而溴化乙锭外排测定是通过荧光测定进行的。通过RT-PCR分析外排泵的转录表达。 LAB显示出抗生素的多抗性概况，此外，乳酸乳球菌（L.）和植物乳杆菌（L.）的乳杆菌显示出更高的溴化乙锭外排表型。金黄色葡萄球菌控制菌株。溴化乙锭的抗性和溴化乙锭外排的表型与L.lmrD的过表达无关。乳酸，或L.lmrA的低表达。植物S和S中的norA。金黄色的。这些发现表明，此外，所分析的外排泵基因，其他未知的冗余机制可能是L的抗生素抗性和溴化乙锭外排表型的基础。乳酸和L。车前草。 LAB中的表型和分子药物多耐药性评估可能会更好地选择用于pozol的发酵剂培养物，并控制广泛的抗生素耐药性和食品变质，以确保健康。

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