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首页> 外文期刊>Food Science & Nutrition >Evaluation of chemical components in Citri Reticulatae Pericarpium of different cultivars collected from different regions by GC–MS and HPLC
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Evaluation of chemical components in Citri Reticulatae Pericarpium of different cultivars collected from different regions by GC–MS and HPLC

机译：通过GC-MS和HPLC评估不同地区不同品种的柑桔果皮的化学成分

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To discriminate the feasible differences and find potential similarities and relationships of Citri Reticulatae Pericarpium (CRP), this work was accomplished by a comprehensive and reliable method using gas chromatography–mass spectrometer (GC–MS) to analyze the volatile oils and high‐performance liquid chromatography (HPLC) simultaneously to determine the contents of five bioactive flavonoids, namely hesperidin, nobiletin, 3,5,6,7,8,3′,4′‐heptamethoxyflavone, tangeretin, and 5‐hydroxy‐6,7,8,3′,4′‐pentamethoxyflavone in 25 batches of CRP samples of 10 cultivars collected from different regions in China. The GC–MS analyses indicated that 98 compounds were successfully identified from the volatile oils obtained and the major constituents of volatile oil are d ‐limonene, γ‐terpinene, α‐pinene, linalool, and myrcene. Even 2‐(methylamino) benzoate was found in all cultivar samples harvested at maturation stage. Under the optimal condition, the quantitative analyses of five bioactive flavonoids were successfully performed by HPLC and hierarchical cluster analysis (HCA). Results showed significant differences among cultivars in the contents of five bioactive flavonoids mentioned earlier. The HCA and GC–MS results provided a convenient approach which might be applied for rapid similarity evaluation and also holds the potential for analysis of compounds present in other plants. Therefore, this work obtained offers scientific basis to control quality and develop medicinal value of the medicinal materials in CRP.
机译：为了区分可行的区别并发现网纹柠檬皮（CRP）的潜在相似性和相关性，这项工作是通过使用气相色谱-质谱仪（GC-MS）分析挥发油和高性能液体的一种全面而可靠的方法来完成的色谱法（HPLC）同时测定5种生物活性类黄酮的含量，橙皮苷，诺比列汀，3,5,6,7,8,3'，4'-庚甲氧基黄酮，橘皮素和5-羟基-6,7,8从中国不同地区收集的10个品种的25个批次的CRP样品中的3'，4'-五甲氧基黄酮。 GC-MS分析表明，从获得的挥发油中成功鉴定出98种化合物，挥发油的主要成分为d-柠檬烯，γ-萜品烯，α-pine烯，芳樟醇和月桂烯。在成熟阶段收获的所有品种样品中甚至都发现了2-（甲基氨基）苯甲酸酯。在最佳条件下，通过HPLC和层次聚类分析（HCA）成功进行了5种生物活性类黄酮的定量分析。结果表明，不同品种之间在上述5种生物活性类黄酮含量上存在显着差异。 HCA和GC-MS结果提供了一种方便的方法，可用于快速相似性评估，也具有分析其他植物中存在的化合物的潜力。因此，所获得的这项工作为控制CRP中的药用质量和开发药用价值提供了科学依据。

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