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首页> 外文期刊>Food Science & Nutrition >Effects of household washing on bacterial load and removal of Escherichia coli from lettuce and ?￠????ready-to-eat?￠???? salads
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Effects of household washing on bacterial load and removal of Escherichia coli from lettuce and ?￠????ready-to-eat?￠???? salads

机译：家庭洗涤对细菌负荷和从生菜和即食食品中去除大肠杆菌的影响。色拉

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Abstract Customer demands for fresh salads are increasing, but leafy green vegetables have also been linked to food-borne illness due to pathogens such as Escherichia coli O157:H7. As a safety measure, consumers often wash leafy vegetables in water before consumption. In this study, we analyzed the efficiency of household washing to reduce the bacterial content. Romaine lettuce and ready-to-eat mixed salad were washed several times in flowing water at different rates and by immersing the leaves in water. Lettuce was also inoculated with E. coli before washing. Only washing in a high flow rate (8 L/min) resulted in statistically significant reductions ( p < .05), ?￠????Total aerobic count?￠???? was reduced by 80%, and Enterobacteriaceae count was reduced by 68% after the first rinse. The number of contaminating E. coli was not significantly reduced. The dominating part of the culturable microbiota of the washed lettuce was identified by rRNA 16S sequencing of randomly picked colonies. The majority belonged to Pseudomonadaceae , but isolates from Enterobacteriaceae and Staphylococcaceaceae were also frequently found. This study shows the inefficiency of tap water washing methods available for the consumer when it comes to removal of bacteria from lettuce. Even after washing, the lettuce contained high levels of bacteria that in a high dose and under certain circumstances may constitute a health risk.
机译：摘要客户对新鲜沙拉的需求正在增加，但是由于诸如O157：H7大肠杆菌等病原体，绿叶蔬菜也与食源性疾病有关。为了安全起见，消费者在食用前经常在水中清洗多叶蔬菜。在这项研究中，我们分析了家庭洗涤减少细菌含量的效率。将长叶莴苣和即食混合色拉在流水中以不同的速率洗涤几次，然后将叶子浸入水中。在洗涤之前，还用大肠杆菌接种了莴苣。仅以高流速（8升/分钟）洗涤会导致统计学上的显着降低（p <.05）。第一次冲洗后，肠杆菌的数量减少了80％，肠杆菌科的数量减少了68％。污染大肠杆菌的数量没有明显减少。通过随机挑选的菌落的rRNA 16S测序鉴定了水洗生菜可培养微生物菌群的主要部分。多数属于假单胞菌科，但也经常发现肠杆菌科和葡萄球菌科的分离株。这项研究表明，从生菜中去除细菌时，消费者无法使用自来水洗涤方法。即使清洗后，生菜中仍含有大量细菌，这些细菌在高剂量下在某些情况下可能构成健康风险。

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