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A comprehensive review on the determination of enzymatic assay and nonenzymatic antioxidant activities

机译：酶法测定和非酶抗氧化活性的测定综述

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This review article presents a comprehensive review pertaining to antioxidants and various assays that determined enzymatic and nonenzymatic antioxidants. Antioxidants have gained attention at the global scale on its prominent beneficial roles that can fight against many chronic infirmities, including cancer and cardiovascular diseases. Many studies have investigated different types of samples, such as medicinal plants, fruits, and vegetables, by using various antioxidant assays. Antioxidants can be grouped into enzymatic and nonenzymatic antioxidants. To date, most studies had looked into nonenzymatic antioxidants due to lack of references on enzymatic antioxidant assays. Therefore, this review article depicts on seven assays of enzymatic antioxidants (superoxide dismutase, catalase, peroxidase, ascorbate peroxidase, ascorbate oxidase, guaiacol peroxidase, and glutathione reductase) and fifteen activities of nonenzymatic antioxidants (total polyphenol, total phenolic acids, total flavonoids, total ascorbic acid, anthocyanin content, DPPH scavenging activity, FRAP assay, hydrogen peroxide scavenging activity, nitric oxide scavenging activity, superoxide radical scavenging activity, hydroxyl radical scavenging activity, phosphomolybdate assay, reducing power, metal ion chelating activity, and β‐carotene), which are described in detail to ease further investigations on antioxidants in future.
机译：这篇综述文章提供了有关抗氧化剂和测定酶促和非酶促抗氧化剂的各种测定方法的综合综述。抗氧化剂以其显着的有益作用而在全球范围内受到关注，这些作用可以对抗许多慢性疾病，包括癌症和心血管疾病。许多研究通过使用各种抗氧化剂分析方法研究了不同类型的样品，例如药用植物，水果和蔬菜。抗氧化剂可分为酶抗氧化剂和非酶抗氧化剂。迄今为止，由于缺乏酶抗氧化剂测定的参考文献，大多数研究已经研究了非酶抗氧化剂。因此，这篇综述文章描述了酶抗氧化剂的七种测定方法（超氧化物歧化酶，过氧化氢酶，过氧化物酶，抗坏血酸过氧化物酶，抗坏血酸氧化酶，愈创木酚过氧化物酶和谷胱甘肽还原酶）和十五种非酶抗氧化剂的活性（总多酚，总酚酸，总黄酮类化合物）总抗坏血酸，花色苷含量，DPPH清除活性，FRAP测定，过氧化氢清除活性，一氧化氮清除活性，超氧自由基清除活性，羟基自由基清除活性，磷钼酸盐测定，还原能力，金属离子螯合活性和β-胡萝卜素） ，对其进行了详细描述，以简化将来对抗氧化剂的进一步研究。

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