• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Frontiers in Cellular and Infection Microbiology >Comparative Genomic and Phylogenetic Analysis of a Shiga Toxin Producing Shigella sonnei (STSS) Strain
【24h】

Comparative Genomic and Phylogenetic Analysis of a Shiga Toxin Producing Shigella sonnei (STSS) Strain

机译：产生志贺毒素的志贺氏菌（STSS）菌株的比较基因组学和系统发育分析

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Shigella strains are important agents of bacillary dysentery, and in recent years S. sonnei has emerged as the leading cause of shigellosis in industrialized and rapidly developing countries. More recently, several S. sonnei and S. flexneri strains producing Shiga toxin (Stx) have been reported from sporadic cases and from an outbreak in America. In the present study we aimed to shed light on the evolution of a recently identified Shiga toxin producing S. sonnei (STSS) isolated in Europe. Here we report the first completely assembled whole genome sequence of a multidrug resistant (MDR) Stx-producing S. sonnei (STSS) clinical strain and reveal its phylogenetic relations. STSS 75/02 proved to be resistant to ampicillin, streptomycin, tetracycline, chloramphenicol, thrimetoprim and sulfomethoxazol. The genome of STSS 75/02 contains a 4,891,717 nt chromosome and seven plasmids including the 214 kb invasion plasmid (pInv) harboring type III secretion system genes and associated effectors. The chromosome harbors 23 prophage regions including the Stx1 converting prophage. The genome carries all virulence determinants necessary for an enteroinvasive lifestyle, as well as the Stx1 encoding gene cluster within an earlier described inducible converting prophage. In silico SNP genotyping of the assembled genome as well as 438 complete or draft Shigella sonnei genomes downloaded from NCBI GenBank revealed that S. sonnei 75/02 belongs to the more recently diverged global MDR lineage (IIIc). Targeted screening of 1131 next-generation sequencing projects taken from NCBI Short Read Archive of confirms that only a few S. sonnei isolates are Stx positive. Our results suggest that the acquisition of Stx phages could have occurred in different environments as independent events and that multiple horizontal transfers are responsible for the appearance of Stx phages in S. sonnei strains.
机译：志贺氏菌菌株是细菌性痢疾的重要病原，近年来，在工业化和快速发展的国家中，S。sonnei已成为志贺菌病的主要原因。最近，在美国的零星病例和疫情中已报道了数种产生志贺毒素（Stx）的S. sonnei和S. flexneri菌株。在本研究中，我们旨在阐明在欧洲分离出的最近鉴定出的产志贺毒素的S. sonnei（STSS）的进化。在这里，我们报告的多药耐药（MDR）Stx产生的S. sonnei（STSS）临床菌株的第一个完整组装的全基因组序列，并揭示其系统发育关系。事实证明，STSS 75/02对氨苄西林，链霉素，四环素，氯霉素，thrimetoprim和磺胺甲恶唑具有抗性。 STSS 75/02的基因组包含一个4,891,717 nt染色体和七个质粒，其中包括214 kb入侵质粒（pInv），具有III型分泌系统基因和相关的效应子。染色体上有23个噬菌体区域，其中包括Stx1转换噬菌体。该基因组携带了肠道侵袭性生活方式所必需的所有毒性决定因素，以及先前描述的诱导型转化原噬菌体中的Stx1编码基因簇。在计算机上对组装的基因组以及从NCBI GenBank下载的438个完整或原始志贺氏菌基因组进行的SNP基因分型显示，S。sonnei 75/02属于最近分化的全球MDR谱系（IIIc）。对来自NCBI的Short Read Archive的1131个下一代测序项目进行的有针对性的筛选证实，只有少数S. sonnei分离株为Stx阳性。我们的结果表明，Stx噬菌体的获得可能在不同的环境中作为独立事件发生，并且多次水平转移是造成S. sonnei菌株Stx噬菌体出现的原因。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号