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首页> 外文期刊>Infection, Genetics and Evolution: Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases >Comparative analysis of the Shiga toxin converting bacteriophage first detected in Shigella sonnei
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Comparative analysis of the Shiga toxin converting bacteriophage first detected in Shigella sonnei

机译：首次在痢疾志贺氏菌中检测到的志贺氏菌毒素转化噬菌体的比较分析

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摘要

Here we report the first complete nucleotide sequence of a Shiga toxin (Six) converting phage from a Shigella sonnet clinical isolate that harbors stx1 operon, first identified in the chromosome of Shigella dysenteriae type 1. The phage named Shigella phage 75/02 Stx displayed Podoviridae morphology. It proved to be transferable to Escherichia coli K-12 strains, and cytotoxicity of the lysogenized strains was demonstrated in Vero cell cultures. Genomic analysis revealed that the prophage genome is circular and its size is 60,875 nt that corresponds to 76 ORFs. The genome of Shigella phage 75/02 Stx shows a great degree of mosaic structure and its architecture is related to lambdoid phages. All the deduced proteins, including the 37 hypothetical proteins showed significant homologies to Six phage proteins present in databases. The phage uniformly inserted into the ynfG oxidoreductase gene framed by phage integrase and antirepressor genes in parental S. sonnei and in the three lysogenized K-12 strains C600, DH5 alpha. and MG1655. The Stx1 prophage proved to be stable in its bacterial hosts and remained inducible. (C) 2015 Elsevier B.V. All rights reserved.
机译：在这里，我们报道了志贺毒素（Six）转化噬菌体的第一个完整核苷酸序列，该噬菌体来自带有志贺氏菌痢疾1型染色体的带有stx1操纵子的志贺氏菌十四醇临床分离株。名为志贺氏菌噬菌体75/02 Stx的噬菌体显示了Podoviridae形态学。它被证明可转移至大肠杆菌K-12菌株，并且在Vero细胞培养中证明了溶菌菌株的细胞毒性。基因组分析表明，该噬菌体基因组是圆形的，其大小为60,875 nt，相当于76个ORF。志贺氏菌噬菌体75/02 Stx的基因组显示出高度的镶嵌结构，其结构与lambdoid噬菌体有关。所有推导的蛋白质，包括37种假设的蛋白质，都与数据库中存在的六种噬菌体蛋白质表现出明显的同源性。噬菌体均匀地插入亲本链球菌和三种溶血的K-12菌株C600，DH5α中由噬菌体整合酶和抗阻遏物基因构成的ynfG氧化还原酶基因。和MG1655。事实证明，Stx1噬菌体在其细菌宿主中稳定并且可以诱导。 （C）2015 Elsevier B.V.保留所有权利。

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