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首页> 外文期刊>Fisheries and Aquatic Sciences >Abalone Haliotis discus hannai Intestine Digests with Different Molecule Weights Inhibit MMP-2 and MMP-9 Expression in Human Fibrosarcoma Cells
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Abalone Haliotis discus hannai Intestine Digests with Different Molecule Weights Inhibit MMP-2 and MMP-9 Expression in Human Fibrosarcoma Cells

机译：不同分子量的鲍鱼鲍鱼肠消化物抑制人纤维肉瘤细胞中MMP-2和MMP-9的表达

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摘要

The abalone Haliotis discus hannai, is one of the economically important species in the fisheries industry. Abalone intestines are one of the by-products of its processing. To investigate its bioactive potential, abalone intestine was digested using an in vitro gastrointestinal (GI) digestion system containing pepsin, trypsin, and -chymotrypsin. The abalone intestine G1 digests (AIGIDs) produced by the GI digestion system were fractionated into AIGID I (> 100 kDa), AIGID II (10-100 kDa), and AIGID III (1-10 kDa) using an ultrafiltration membrane system. Of the three digests, AIGID II and AIGID III exhibited inhibitory effects against matrix metalloproteinase-2 and -9 (MMP-2, MMP-9) in HT1080 human fibrosarcoma cells. Both fractions potently inhibited gelatine digestion by MMP-2 and MMP-9 treated with phorbol 12-myristate 13-acetate (PMA) and migration of HT1080 cells in dose dependently. Furthermore, AIGID II and III attenuated expression of p65, a component of nuclear transcription factor kappa B. These results indicate that of the abalone intestine digests inhibit MMP-2 and MMP-9. Thus, the AIGIDs or their active components may have preventive and therapeutic potential for diseases associated with MMP-2 and MMP-9 activation in fibrosarcoma cells.
机译：鲍鱼Haliotis discus hannai，是渔业中经济上重要的物种之一。鲍鱼肠是其加工的副产品之一。为了研究其生物活性潜力，使用包含胃蛋白酶，胰蛋白酶和胰凝乳蛋白酶的体外胃肠道（GI）消化系统消化鲍鱼肠。使用超滤膜系统将由GI消化系统产生的鲍鱼肠G1消化物（AIGID）分为AIGID I（> 100 kDa），AIGID II（10-100 kDa）和AIGID III（1-10 kDa）。在这三种消化物中，AIGID II和AIGID III在HT1080人纤维肉瘤细胞中表现出对基质金属蛋白酶2和-9（MMP-2，MMP-9）的抑制作用。这两个部分均有效地抑制了用佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA）处理的MMP-2和MMP-9的明胶消化，并且剂量依赖性地抑制了HT1080细胞的迁移。此外，AIGID II和III减弱了p65的表达，p65是核转录因子kappa B的组成部分。这些结果表明，鲍鱼肠消化物抑制MMP-2和MMP-9。因此，AIGID或其活性成分可对纤维肉瘤细胞中与MMP-2和MMP-9活化有关的疾病具有预防和治疗潜力。

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