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首页> 外文期刊>International journal of molecular medicine >Anti-allergic effects of a nonameric peptide isolated from the intestine gastrointestinal digests of abalone (Haliotis discus hannai) in activated HMC-1 human mast cells
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Anti-allergic effects of a nonameric peptide isolated from the intestine gastrointestinal digests of abalone (Haliotis discus hannai) in activated HMC-1 human mast cells

机译：从鲍鱼肠胃消化物中分离的非异构肽对激活的HMC-1人肥大细胞的抗过敏作用

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摘要

The aim of the present study was to examine whether the intestine gastrointestinal (GI) digests of abalone [Halioti s discus hannai (H. discus hannai)] modulate inflammatory responses and to elucidate the mechanisms involved. The GI digests of the abalone intestines were fractionated into fractions I (>10 kDa), 11 (5-10 kDa) and III (<5 kDa). Of the abalone intestine GI digests (AIGIDs), fraction III inhibited the passive cutaneous anaphylaxis (PCA) reaction in mice. Subsequently, a bioactive peptide [abalone intestine GI digest peptide (AIGIDP)] isolated from fraction III was determined to be 1175.2 Da, and the amino acid sequence was found to be PFNQGTFAS. We noted that the purified nonameric peptide (AIGIDP) attenuated the phorbol-12-myristate 13-acetate plus calcium ionophore A23187 (PMACI)-induced histamine release and the production of pro-inflammatory cytokines, such as tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-1 beta and IL-6 in human mast cells (HMC-1 cells). In addition, we also noted that AIGIDP inhibited the PMACI-induced activation of nuclear factor-kappa B (NF-kappa B) by suppressing I kappa B alpha phosphorylation and that it suppressed the production of cytokines by decreasing the phosphorylation of JNK. The findings of our study indicate that AIGIDP exerts a modulatory, anti-allergic effect on mast cell-mediated inflammatory diseases.
机译：本研究的目的是检查鲍鱼肠胃（GI）消化物（Halioti s discus hannai（H. discus hannai））是否能调节炎症反应并阐明其相关机制。将鲍鱼肠的消化消化物分为I（> 10 kDa），11（5-10 kDa）和III（<5 kDa）级分。在鲍鱼肠GI消化液（AIGID）中，级分III抑制了小鼠的被动皮肤过敏反应（PCA）。随后，从级分III分离的生物活性肽[鲍鱼肠GI消化肽（AIGIDP）]被确定为1175.2Da，并且发现氨基酸序列为PFNQGTFAS。我们注意到，纯化的非异构肽（AIGIDP）减弱了佛波12-肉豆蔻酸酯13-乙酸酯加钙离子载体A23187（PMACI）诱导的组胺释放和促炎细胞因子的产生，例如肿瘤坏死因子-α（TNF- α），白细胞介素（IL）-1 beta和IL-6在人类肥大细胞（HMC-1细胞）中的分布。此外，我们还注意到AIGIDP通过抑制IκBα磷酸化来抑制PMACI诱导的核因子κB（NF-κB）的活化，并且它通过降低JNK的磷酸化来抑制细胞因子的产生。我们研究的结果表明AIGIDP对肥大细胞介导的炎性疾病具有调节性，抗过敏作用。

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