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Extracellular Electrophysiological Measurements of Cooperative Signals in Astrocytes Populations

机译：星形胶质细胞群体中合作信号的细胞外电生理测量。

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Astrocytes are neuroglial cells that exhibit functional electrical properties sensitive to neuronal activity and capable of modulating neurotransmission. Thus, electrophysiological recordings of astroglial activity are very attractive to study the dynamics of glial signaling. This contribution reports on the use of ultra-sensitive planar electrodes combined with low noise and low frequency amplifiers that enable the detection of extracellular signals produced by primary cultures of astrocytes isolated from mouse cerebral cortex. Recorded activity is characterized by spontaneous bursts comprised of discrete signals with pronounced changes on the signal rate and amplitude. Weak and sporadic signals become synchronized and evolve with time to higher amplitude signals with a quasi-periodic behavior, revealing a cooperative signaling process. The methodology presented herewith enables the study of ionic fluctuations of population of cells, complementing the single cells observation by calcium imaging as well as by patch-clamp techniques.
机译：星形胶质细胞是神经胶质细胞，其表现出对神经元活动敏感的功能性电特性并能够调节神经传递。因此，星形胶质活动的电生理记录对于研究神经胶质信号的动力学非常有吸引力。该贡献报告了超低灵敏度平面电极与低噪声和低频放大器的组合使用，这些电极能够检测由从小鼠大脑皮层分离的星形胶质细胞的原代培养物产生的细胞外信号。记录的活动的特征是由离散信号组成的自发突发，信号速率和幅度发生明显变化。弱信号和零星信号变得同步，并随时间演变为具有准周期性行为的高幅度信号，从而揭示了协作信号过程。本文介绍的方法能够研究细胞群体的离子涨落，通过钙成像以及膜片钳技术补充单细胞的观察。

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